【作者】 王静；

【导师】 邢向晖；

【作者基本信息】 山东中医药大学 ， 中医儿科学， 2004， 硕士

【摘要】 本文旨在运用中医和中西医结合理论方法探讨小儿难治性肾病综合征的病因病机和临床征候特点，针对西医治疗难治性肾病综合征的常用药物环磷酰胺对性腺的毒副作用，通过实验研究和临床观察，明确补肾健脾方对性腺的影响。实验研究了环磷酰胺及环磷酰胺与补肾健脾方合用对大鼠性腺和性激素的影响，初步探讨了其作用机理。动物实验表明：补肾健脾方和环磷酰胺联合使用与单独使用环磷酰胺相比，二者联合使用可使大鼠的血清睾酮含量明显高于环磷酰胺致性腺损伤大鼠的血清睾酮含量，同时具有降低卵泡刺激素、促黄体生成素含量，并调节性激素水平趋向正常的作用。临床观察结果也显示，29例男性患者中，补肾健脾方与环磷酰胺配合使用，结果无一例性腺损伤。分析认为补肾健脾方可补肾健脾保护和促进生殖机能，并可能通过促进下丘脑-垂体-性腺轴的组织形态学改变和调节其内分泌、以及清除自由基，发挥对抗或减轻环磷酰胺的性腺毒性的作用。实验研究表明，补肾健脾方与环磷酰胺合用治疗难治性肾病综合征，可以提高疗效，且可在一定程度上减轻环磷酰胺导致的性腺损害，为临床应用提供了一定的实验依据。更多还原

【Abstract】 The aim of this thesis is to study the characteristics of pathogenesis and ckinical sign of baby’ s refractory nephritic syndrome by means of theories of Traditional Chinese Medicine and the combination of Chinese Material Medica(CMM) with West medicine and to study the Bushen-Jianpi formula’ s effects on sexual gland by virtues of clinical observation and experiments. To overcome the toxic side effects of ordinary medicine cytoxan(CTX) in treating with refractory nephritic syndrome on sexual gland, the experiments were designed and carried out to modify the side effects on sexual gland by making use of Bushen-Jianpi CMM. The effect of CTX and the co-effects of CTX combining with Bushen-Jianpi CMM on sexual gland were measured and the acting mechanism of CMM were preliminarily analyzed as well.The tests showed that the combination of Bushen-Jianpi CMM formula with CTX can raise the content of testosterone in blood serum of Rat relative to that treated with CTX, decrease the contents of follicle-stimulating hormone(FSH) and Luteinizing hormone releasing factor, and adjust the level of sex hormone to ordinary states.The results of clinical observation and experiments showed that in 29 cases of the male disease, no one of their sexual gland were harmed by treating with CTX combining with Bushen-Jianpi CMM.The analysis on the results of the experiments show that Bushen-Jianpi CMM are able to prevent reproduction ability from being harmed by CTX and promote reproduction ability as well as may have effects on the organization morphology and endocrine through the hypothalamus-hypophysis-sexual gland axis. Bushen-Jianpi CMM formula can eliminate free radical and resist or reduce the toxicant effects of CTX on sexual gland of Rat.The experiments also show that the combination of CTX with Bushen-Jianpi CMM formula can promote the efficiency in dealing with refractory nephritic syndrome, and decrease the harm of CTX on sexual gland of Rat, which provides the experiment base on clinical applications.更多还原