【作者】 郭继龙；

【导师】 王世军；

【作者基本信息】 山东中医药大学 ， 中西医结合基础， 2004， 硕士

【摘要】 目的 研究收涩中药乌梅、椿皮、金樱子、五昧子、五倍子、赤石脂、禹余粮抑制血管生成及抗肿瘤生长的作用及其机制。方法 建立鸡胚绒毛尿囊膜血管模型，观察药物对鸡胚绒毛尿囊膜血管生成的影响。采用小鼠移植肿瘤模型，研究药物对肿瘤生长的影响；应用免疫组织化学方法，检测肿瘤组织相关第八因子抗原(Ⅷ-RAg)和基质金属蛋白酶9(MMP-9) 的表达，通过医用图像处理软件测定肿瘤组织的微血管密度(MVD)。结果 收涩药物金樱子、五味子、五倍子、乌梅、赤石脂、椿皮对鸡胚绒毛尿囊膜新生血管数目有一定抑制作用；乌梅、椿皮对小鼠皮下移植肉瘤有明显抑制作用(P<0． 05) ；血管密度椿皮组明显低于空白和阳性对照组(P<0． 05) ；乌梅、椿皮组相对于空白对照组MMP-9呈低表达(P<0． 05) 。结论 收涩中药椿皮对鸡胚绒毛尿囊膜血管及肿瘤血管生成有一定的抑制作用，可以抑制移植S180肉瘤的生长及MMP-9的表达。更多还原

【Abstract】 Objective To investigate the effect on angiogensis as well as tumor growth of the traditional Chinese medical astringent, such as, Fructus Mume, Cortex Ailanthi, Cherokee Rose Fruit, Chinese Magnoliavine, Chinese Gall, Red Halloysite, Limonitum, and the mechanism of it.Method The chicken chorioallantoic membrane (CAM) angiogensis model was used to study the effect of these medical herbs on angiogensis. a model of transplanting tumor in mice was used to study the effect of these medical herbs on tumor growth. The expression of VⅢ-RAg and matrix metalloproteinase-9 (MMP-9) in tumor tissue were examined with immunohistochemical method. The microvessel density (MVD) of the tumour tissue was also measured with the Image Pro Plus medical image system. Result Cherokee Rose Fruit, Chinese Magnoliavine, Fructus Mume, Red Halloysite, Cortex Ailanthi can inhibit the angiogensis of CAM. Fructus Mume and Cortex Ailanthi can obviously inhibit the growth of sarcoma (S180) transplanted subcutaneously in mice (P<0. 05). There were fewer vessels in sarcoma tissue in Cortex Ailanthi group than in control group and CTX group(P<0.05). The expression of MMP-9 was lower in Cortex Ailanthi group and Fructus Mume group than in control group(P<0. 05). Conclusion Cortex Ailanthi can inhibit angiogensis in CAM and tumor tissue, and can also suppress the growth of sarcoma (S180) transplanted in mice and the expression of MMP-9 in the sarcoma tissue.更多还原