【作者】 刘伦；

【导师】 朱美玲； 王明丽； 陈逖；

【作者基本信息】 安徽医科大学 ， 眼科学， 2003， 硕士

【摘要】 目的 利用碱烧伤制备兔角膜新生血管模型，在新生血管形成的各时段检测角膜基质金属蛋白酶-2(MMP-2)及其组织型抑制物(TIMP-2)的表达，观察早期新鲜羊膜移植对MMP-2、TIMP-2表达的影响和抑制新生血管的效果。探讨早期新鲜羊膜移植抑制角膜新生血管可能的机理。 方法 40只新西兰白兔，以1mol／L的氢氧化钠碱烧伤双眼制备角膜新生血管模型，伤后右眼立即行羊膜移植术，右眼为羊膜移植组即A组(n=40)，左眼作为烧伤对照组即B组(n=40)，另有6只兔作为正常对照即C组(n=12)。术后密切观察并记录角膜新生血管形成和生长状况，测量新生血管长度，计算新生血管面积。术后第1、3、7、14、28天处死动物，取下角膜，分别行HE染色、MMP-2和TIMP-2多克隆抗体免疫组化染色、逆转录聚合酶链反应(RT-PCR)半定量检测MMP-2和TIMP-2的表达。 结果 所有碱烧伤的角膜均有新生血管出现，正常对照组无新生血管出现。羊膜移植组新生血管的长度和面积均明显小于烧伤对照组(P＜0.05)，MMP-2和TIMP-2在正常角膜中微弱表达，烧伤后第1天MMP-2和TIMP-2表达即开始增强，第3天达高峰，第7天仍然较高，之后开始下降，羊膜移植组与烧伤对照组比较，各时段MMP-2表达减弱，而TIMP-2的表达增强，差异有显著性(P＜0.05)。 结论 在角膜新生血管形成期间，MMP-2和TIMP-2表达均增强，早期新鲜羊膜移植能抑制角膜新生血管生长，其机制可能是通过调节MMP-2与TIMP-2间的平衡来完成。更多还原

【Abstract】 Objective To investigate the anti-angiogenesis effects and possible mechanism of amniotic membrane transplantation(AMT) on corneal neovascularization(CNV). We examined the expression of matrix metalloproteinase-2(MMP-2) and its inhibitor (TIMP-2) during the course of alkali burn-induced corneal in a rabbit model with or without AMT.Methods Corneal neovascularization model of 40 New Zealand rabbits were established by means of alkali burn. Amniotic membrane transplantation were performed on right eyes immediately after burn(group A), The left eyes were uncovered with amniotic membrane(group B). Another 6 rabbits were in control group(group C). The occurrence and development of CNV were observed attentively. The length and the area of CNV were measured and calculated. The rabbits were killed on the 1st, 3rd, 7th, 14th, 28th day, the corneas were removed for histopathologic study, immuneo-histochemistry study and RT-PCR study.Results CNV can be observed on all of the corneas suffered from alkali bum, while cannot be seen on control group. The length and the area of CNV in group A was significantly smaller compared with that in group B (P<0.05). The expression of MMP-2 and TIMP-2 in untreated corneas were weak or absent. Increased expression was found immediately on the 1st day, the highest expression was found on the 3 rd day, strong expression could also be observed on the 7th day. The expression was decreased after that time. The expression of MMP-2 in group A were lower than that in group B during the course, while the expression of TIMP-2 in group A were higher than that in group B(P<0.05). Conclusion During the course of CNV formation, the expression of MMP-2 andTIMP-2 were increased. Fresh amniotic membrane transplantation could significantly inhibit CNV, which mechanism was supposed to be through the regulation of MMP-2 and TIMP-2.更多还原