【作者】 方士英；

【导师】 李俊；

【作者基本信息】 安徽医科大学 ， 药理学， 2004， 硕士

【摘要】 本文采用了化学和免疫性小鼠肝损伤模型，分别从整体和细胞水平上研究了安徽境内新发现的古尼虫草多糖的护肝作用，并从免疫调节和细胞因子水平变化的角度，研究并分析了虫草多糖对免疫性肝损伤保护作用的部分机制。 1、虫草多糖对CCl₄诱导小鼠化学性肝损伤的影响 虫草多糖(150、250、500 mg·kg^-1)ig给药能显著降低CCl₄(100 mg·kg^-1，ip)诱导的急性肝损伤引起的血浆中升高的ALT、AST和肝匀浆中升高的MDA水平，并增强肝匀浆中SOD的活性，病理学检验显示对肝细胞坏死具有一定的保肝作用。提示，虫草多糖对CCl₄诱导的小鼠化学性肝损伤具有明显的保护作用。 2、虫草多糖对D-Gal诱导小鼠化学性肝损伤的影响 虫草多糖(150、250、500 mg·kg^-1)ig给药能显著降低D-Gal(800mg·kg^-1，ip)诱导的急性化学性肝损伤引起的血浆中升高的ALT、AST和肝匀浆中升高MDA，并增强肝匀浆中SOD的活性，病理学检验显示对肝细胞的变性具有一定的保肝作用。提示，虫草多糖对D-Gal引起的小鼠化学性肝损伤具有明显的保护作用。女徽医科人学硕1:学位论文 3、虫草多糖对BCG+LPS诱导的免疫性肝损伤的影响 在尾静脉iv BCG+LPS成功诱导免疫性肝损伤模型的基础上，采用虫草多糖（150、250、500 nlg·kg一，）19给药能显著降低BeG+LPs诱导的免疫性肝损伤引起的血浆中升高的ALT、AST和肝匀浆中升高的MDA水平，并增加肝匀浆中SOD的活性。病理组织学检验显示，能减轻肝脏炎细胞浸润和坏死程度。提示，虫草多糖对 BCG+LPs诱导的免疫性肝损伤也具有明显的保护作用。 弓、虫草多糖对BCG+LPS诱导的免疫性肝损伤影响的机制 虫草多糖（150、250、500 mg·kg一，）ig给药能显著降低Bcc+LPs诱导的免疫性肝损伤升高的肝脏中TNF一。和IL一1含量。提示，虫草多糖对免疫性肝损伤的保护作用可能与其平衡细胞因子产生、调节机体免疫功能有关。更多还原

【Abstract】 The assay used the chemical and immunological experimental liver injury models to investigate the effect of cordyceps polysaccharides from cordyceps gunni var minor nov in Anhui provice on liver injury on the level of cell and body respectively. It also analysed the mechanism of its protective effect on immunological hepatic injury. The main results as follows:1. Effect of cordyceps polysaccharides on CCl4-induced chemical liver injury models.Cordyceps polysaccharides( 150, 250s 500 mg kg-1, ig ) could significantly reduce elevated ALT, AST activities in plasma and MDA content of liver homogenates,and enhaced activity of SOD in liver in CCl4(1OO mg kg-1 , ip)-induced chemical liver injury mice. The pathology resul ts of liver also showed cordyceps polysaccharides had liver protection to a certain extent. All of these suggested cordyceps polysaccharides hadan action of protection from CC14 -induced chemical liver injury.2. Effect of cordyceps polysaccharides on D-Gal-induced chemicl liver injury models.With the same dose and methods ( 150, 250, 500 mg kg-1, ig ) the experiment showed that cordyceps polysaccharides had the same protective effects on hepatic injury caused by D-Gal as it caused by CC14,which suggested cordyceps polysaccharides had an action of protection from D-Gal-induced chemical liver injury.3. Effect of cordyceps polysaccharides on BCG+LPS-induced immunological liver injury models.On the base of models of BCG+LPS-induced immunological liver injury, cordyceps polysaccharides ( 150, 250, 500 ing ’ kg’’, ig ) had the same protective effect on immunological hepatic injury as those caused by the above two drugs. It suggested cordyceps polysaccharides had an action of protect ion from BCG+LPS-induced immunological liver injury.4. Mechanisms of action of cordyceps polysaccharides on immunological liver injuryDifferent dose of cordyceps polysaccharides (150, 250,500 mg kg-1, ig) could significantly inhibited TNF-ccand IL-1 production in liver. It suggested that the protective action of cordyceps polysaccharides on immunological liver injury was possibly related to its equilibrum on cytokines production and modulation of immunological function.更多还原