【作者】 高登辉；

【导师】 魏伟；

【作者基本信息】 安徽医科大学 ， 药理学， 2004， 硕士

【摘要】 目的：探讨白芍总苷(total glucosides of paeony，TGP)对人白蛋白致大鼠免疫性肝纤维化模型的作用及其部分机制。 方法：建立人白蛋白致大鼠免疫性肝纤维化模型，分光光度法检测血清中丙氨酸氨基转移酶(alanine aminotransferase，ALT)、天门冬氨酸氨基转移酶(aspartateaminotransferase，AST)、一氧化氮(nitric oxide，NO)、脯氨酸肽酶(prolidase，PLD)水平和肝匀浆中羟脯氨酸(hydroxyproline，Hyp)、丙二醛(malondiadehyde，MDA)、超氧化物歧化酶(superoxide dismutase，SOD)、谷胱甘肽过氧化物酶(glutathioneperoxidase，GSH-px)含量，放免法检测血清中透明质酸(hyaluronic acid，HA)和Ⅲ型前胶原(procollagen Ⅲ，PCⅢ)水平，ELISA法检测血清中血清转化生长因子β(transforming growth factor-β，TGF-β)水平。HE染色法对肝脏组织作病理检查。 结果：TGP可明显减轻人白蛋白所致的肝纤维化程度，不仅降低肝匀浆Hyp含量和血清HA和PCⅢ含量，还可减轻肝脏纤维化的病理组织学改变。虽然TGP对人白蛋白致肝纤维化大鼠的肝功能和A／G值变化无明显影响，但TGP能改善肝纤维化大鼠肝脏的氧化状态，减少MDA的产生，提高抗氧化酶的活性，同时发现TGP可减少致肝纤维化细胞因子TGF-β的产生。 结论：TGP具有明显抗大鼠免疫性肝纤维化的作用，其机制可能与清除自由基、提高抗氧化酶活性和减少TGF-β的生成等作用有关。更多还原

【Abstract】 Objective To study the effects and mechanisms of total glucosides ofpaeony (TGP) on immunological hepatic fibrosis induced by human albumin in rats . Methods The model of immunological hepatic fibrosis induced by human albumin was prepared. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) , prolidase (PLD), nitric oxide(NO) in serum, hydroxyproline(Hyp) content, malondiadehyde(MDA) content, superoxide dismutase(SOD) and glutathione peroxidase(GSH-px) activities in liver homogenate were assayed by spectrophotometry; Serum procollagen type III (PC III) and hyaluronic acid (HA) was determined by radioimmunoassays. The level of transforming growth factor- (TGF- )was determined by ELISA method. Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light microscope. Results: Histological results showed that TGP improved the human albumin -induced alterations in the liver structure, alleviated lobular necrosis and significantly lowered collagen content. The anti-fibrotic effect of TGP also confirmed by decreased serum content of HA and PCIII in TGP-treated group. Moreover, the treatment with TGP effectively reduced the hydroxyproline content in liver homogenate. However, the level of ALT and AST increased in fibrotic rat but had no significance compared with normal control, whereas the ratio of A/G decreased also had no significance. TGP had no effect on level of ALT ,AST and the ratio of A/G Furthermore, TGP treatment significant blocked the increase in MDA and NO, associated with a partially elevation in liver total antioxidant capacity including SOD and GSH-px. Meanwhile, TGP treatment significant decreased the production of TGF- 3 .Conclusion TGP have beneficial effects on hepatic fibrosis in rats by inhibition of collagen synthesis, decreasing oxidative stress and TGF- production.更多还原