【作者】 陈森；

【导师】 王爱玲； 余元勋；

【作者基本信息】 安徽医科大学 ， 内科学， 2004， 硕士

【摘要】 目的：通过检测急性心肌梗死患者及对照人员的β纤维蛋白原基因-455G／A多态性，探讨该多态性与急性心肌梗死发病的关系，寻找急性心肌梗死发病的遗传性危险因素。对象：急性心肌梗死患者40例，均符合世界卫生组织关于急性心肌梗死的诊断标准。对照组40人(排除有冠心病、缺血性脑卒中及外周血管血栓疾病者)。方法：β纤维蛋白原-455基因多态性分析：(1)碘化钾法提取人基因组DNA；(2)多聚酶链反应(PCR)扩增目的基因片段；(3)扩增目的基因片段加Hae Ⅲ限制性内切酶水浴消化；(4)酶切产物经3％琼脂糖凝胶电泳分离后，紫外灯下检测酶切结果。SPSS11.5软件分析数据。结果：(1)急性心肌梗死组中β纤维蛋白原-455A等位基因频率显著高于对照组。(2)急性心肌梗死组中β纤维蛋白原-455GA和AA基因型携带者频率显著高于对照组。(3)在β纤维蛋白原-455三种基因型之间，高血压病、糖尿病发病率无显著性差异。(4)在β纤维蛋白原-455三种基因型之间，空腹血清甘油三脂、血糖及总胆固醇浓度无显著性差异。(5)急性心肌梗死组中合并糖尿病患者的发病频率显著高于对照组。(6)急性心肌梗死组中空腹甘油三脂及平均血糖浓度显著高于对照组。(7)多元logistic回归分析发现，性别、血糖及β纤维蛋白原-455AA+GA基因型对急性心肌梗死的发病有明显影响作用。结论：(1)β纤维蛋白原-455A等位基因可能是急性心肌梗死发病的遗传性危险因素。(2)β纤维蛋白原-455G／A多态性可能与高血压病和糖尿病的发病无关。(3)β纤维蛋白原-455G／A多态性可能与甘油三脂及总胆固醇代谢无关。(5)急性心肌梗死发病与性别、血清甘油三脂和血糖水平有关。更多还原

【Abstract】 Objectives: To analyze the frequency of -fibrinogen gene -455 genotypes in acute myocardial infarction (AMI) group and control group. To investigate wether the -455G/A polymorphisms were associatied with the risk of AMI. To demonstrate wether possession of the -455A allele is a genetic risk of AMI. Subject and Methods: Forty patients suffered from acute myocardial infarction within one week, as defined by World Health Organization criteria. And forty control subjected were recruited. The participators in cnotrol group had no histories of coronary heart disease, ischemic stroke and peripheral arterial disease. The -455 polymorphism in the -fibrinogen gene were detected by polymerase chair reaction amplication of fragments of DNA by using specific oligonucleotide primers in a thermal cycler. Polymerase chain reaction products were subjected to 3 hours digestion by 4U HaeIII restriction enzymes at 37 C. Restricted DNA products were then separated by useng 3% agarose gel electrophoresis and visuslized by UV light. Interactions between these changes and other factors were analyzed with SPSS statistical software 11.5. Result: (1) There were signiferences diferrences in fibrinogen -455A allele distributions between AMI and in control groups, the frequence of -455A allele were higher in AMI group than control group. (2) There were signiferences diferrences in fibrinogen -455 genotype distributions between AMI and control groups, the frequence of -455AA and GG genotypes in AMI group were higher than that in control group. (3) There were no signiferences diferrences in hypertension and diabates groups in three fibrinogen -455 genotypes. (4) There were no signiferences diferrences in free plasma blood sugar, total cholesterol and triglyceride levels in three fibrinogen -455 genotypes. (5) Levels of sugar and blood triglyceride were higher in patients than that in control subjects. (5) fibrinogen-455GA and AA genotypes were contribute with acute myocardial infarction. (6)Sex and blood sugar were contribute with acute myocardial infarction. Conclusion: (1) Fibrinogen β -455G/A polymorphisms is a genetic risk factor of acute myocardial infarction. (2) Fibrinogen β -455G/A polymorphisms have no associations with hypertension and diabetes. (3) Fibrinogen 3 -455G/A polymorphisms have no associations with the metabolism of sugar, cholesterol and triglyceride. (4) Sex, blood sugar and triglyceride have associations with AMI.更多还原