【作者】 吴金辉；

【导师】 张西正；

【作者基本信息】 中国人民解放军军事医学科学院 ， 生物医学工程， 2004， 硕士

【摘要】 本文根据国内外组织工程化培养生物反应器研究现状，利用有限元方法分析了旋转壁式生物反应器内部流场情况和剪应力分布情况，探讨了生物反应器与培养物之间的传质过程以及脉冲压力对传质能力的影响。并在计算机仿真和理论分析的基础上，研制了一种具有内循环的灌流旋转壁式生物反应器，对其性能进行了相关实验和检测。 通过理论分析给出了旋转壁式生物反应器中培养物受到剪应力以及流动轨迹的矢量图。建立了旋转壁式生物反应器内部流场的有限元模型，使用ANSYS有限元分析软件计算了培养物表面剪应力大小和分布，描绘了生物反应器空间流场的速度矢量云图，并与理论分析比较，结果取得了较好的一致性。 通过对生物反应器与培养物之间传质过程的分析，得出了影响传质能力的关键因素，研究表明适当脉冲压力对传质能力有促进作用，并可以用脉冲压力实现高传质能力生物反应器的设计。 基于理论分析和有限元仿真，设计了一台具有内循环的、在线供气供液的并带有脉冲加压装置的旋转壁式生物反应器系统。生物反应器性能测试实验的结果表明：在生物反应器中培养14天后的细胞／羟基磷灰石支架材料以及细胞／松质骨支架材料，其细胞的增殖和形态都明显优于在5％CO₂孵箱中培养的同类材料。更多还原

【Abstract】 Based on the development of the bioreactor used to the culture of tissue engineering in the nation and the overseas, this thesis analyzed the ulterior fluid field and the shearing stress distribution of the rotating wall bioreactor by using the finite element method, discussed the mass transfer process between the bioreactor and the culture and the infection of the impulse press. Based on the computer simulation and the theoretically analysis, we have designed and manufactured a rotating wall perfusion bioreactor system with interior circulation, and tested its capability with correlative experimentations.Through the theoretically analysis the vector figures of the shearing stress and the moving track of the culture within the rotating wall bioreactor have been given out. We also built a finite element model about the rotating wall bioreactor interior fluid field, calculated the shearing stress’ values and the distribution status of the cultures, described the fluid velocity vector figure of the bioreactor space, and compared with the theoretically analysis results, the research results were primely consistent with them.We also analyzed the mass transfer process between the bioreactor and the culture, found some key factors which evidently affected the ability of the mass transfer. We proved that proper impulse press could enhance the mass transfer, and we could design a kind of high mass transfer bioreactor by the impulse press.Based on the theoretically analysis and the finite element simulation, we have designed a rotating wall bioreactor system with interior circle, offering gas and medium on-line and impulse press device. This bioreactor can offer a culture space about 700ml. The bioreactor can culture micro-carrier and cell/tissue scaffold.The testing results of the bioreactor system indicated that our bioreactor has a better ability to culture the scaffold with cells: hydroxy-phosphorite scaffolds and cancellous bone with cells which cultured our bioreactor system proliferated very markedly; cells cultured in it about 14 days have better modality than cultured in the 5%Researching of the bioreactor used to the culture of tissue engineering _ Abstractincubater.更多还原