【作者】 陆春霞；

【导师】 韦鹏霄；

【作者基本信息】 广西大学 ， 作物遗传育种， 2004， 硕士

【摘要】 本研究论文主要是通过对百合进行系统的组织培养试验，建立起良好的百合二倍体体细胞无性系，并结合辐射诱变和秋水仙素溶液处理方法进行多倍体的诱导试验，为今后的百合组织培养与多倍体研究提供重要的参考依据，试验结果表明： 1、不同基因型材料的诱导分化能力不同，兰州百合的诱导分化率为51.65％，平均生成的小鳞茎数为5.87；龙牙百合的诱导分化率为22.22％，平均生成的小鳞茎数为2.15。 2、同一材料不同位置的鳞片其诱导能力也有所差异，分别表现为，兰州百合：内层＞中层＞外层；龙牙百合：外层＞中层＞内层。 3、不同激素种类与浓度配比对叶片的诱导分化结果不同，当2，4-D浓度为1.5-2.5mg．L^-1时，有利于愈伤组织的诱导；当激素为0.5 mg．L^-16-BA和0.5 mg．L^-1NAA的组合时，有利于芽的分化。 4、激素组合KT_0.5mg．L^-1+BA_0.5mg．L^-1对台湾新铁炮百合原球茎的增殖效果较好。 5、使用单因子的生长素NAA或IAA不利于百合的继代增殖。 6、低浓度的KT有利于兰州百合的继代增殖；而较高浓度的6-BA对台湾新铁炮百合增殖效果较好。 7、较低浓度范围(0.3-0.6mg．L^-1)的多效唑对百合的增殖有一定的促进作用。 8、不同的激素组合对百合芽的继代增殖效果不同，以6-BA_0.5mg．L^-1+NAA_0.1mg．L^-1+MET_0.5mg．L^-1+KT_0.5mg．L^-1的激素配比对百合的继代效果最广西大学硕士学位论文百合组织培养与多倍体研究好。 9、两种生长素的配合使用比使用单一生长素更有利于百合的生根壮刁闷卜田。 10、较低浓度范围（0 .6一0.smg.L一’）的多效哩对百合的生根壮苗有一定的促进作用。 11、浓度为2.omg.L一’ABTI号生根粉对兰州百合的生根壮苗效果最好。 12、不同基因型材料对辐射的敏感性不同，兰州百合的辐射敏感性要大于台湾新铁炮百合。 13、秋水仙素溶液的诱变效果因基因型而异，当秋水仙素浓度为0.7%，处理天数为5天时，诱变效果最好.其中兰州百合的变异率为40%，台湾新铁炮百合的变异率为30%。 14、不同基因型材料的诱变效果因秋水仙素溶液处理的方法不同而存在差异，兰州百合适合使用浸泡法，台湾新铁炮百合适宜用棉球覆盖法。 15、百合四倍体与二倍体的气孔保卫细胞数目及大小之间的差异显著。 16、细胞学鉴定二倍体兰州百合和台湾新铁炮百合的染色体数为24（Zn=ZX=24，x=12），而其四倍体的染色体数为45（4n二4X=48，x=24）。更多还原

【Abstract】 In this paper we studied tissue culture of Lily systematically , established a good diploid system, used radiation mutagenesis and colchicines liquid treatments to induce tetraploid plants, in order to offer significant referenced datum for the future. The results showed:1, There were different frequencies of buds induced and differentiation with different genotype varieties, the frequency of buds differentiation with Lilium davidii var. unicalar Cotton was 51.65%, the average of induced bulblets was 5.87; that of Lilium brownii E E. Brown var. viridulum Baker was 22.22% and 2.15.2, Different part of a bud scale gave different responses to the effect of induction with the same varieties. The inducting frequencies of inner scales with Lilium davidii var. unicalar Cotton was the highest, middle scales was the second ,and outer scales was the lowest; But the result of Lilium brownii F. E. Brown var. viridulum Baker was quite the contrary.3, There were different hormone categories and concentration combinations in leaves’ ability of induction and differentiation, when the concentration range of 2, 4-D was 1.5-2.5mg.L ’ ,it’s good for callus induction; when the medium combine with 0.5 mg.L-16-BA and 0.5 mg.L-1NAA were more profit for bud induction.4 , The optimal hormone combination for embryoids multiplication of Lilium longiflorum was 0.5mg.L-1KT and 0.5 mg.L-16-BA.5, Choosing one-factor auxin was adverse to buds subculture multiplication of Lily, for example NAA or IAA.6, Low concentration of KT was of advantage to buds subculture multiplication of Lilium davidii var. unicalar Cotton; and higher concentration of 6-BA was better to Lilium longiflorum’s multiplication.7, Lower concentration range of MET (0.3-0.6mg.L-1) can accelerate budssubculture multiplication of lily.8, Different hormone combinations gave different responses to the effect of buds subculture multiplication. The treatment with BA0.5mg.L-1+NAA0.1mg.L-1+MET o.5mg.L-1+KTo.5 mg.L"1 showed better effect than others.9, The medium combine with two auxins were more propitious to root inducing and sound seedling of Lily than that of one-factor auxin.10 , Lower concentration range of MET(0.6-0.8mg.L-1) can accelerate root inducing and sound seedling of Lily.11, The best effect concentration of ABT1 on root inducing and sound seedling with Lilium davidii var. unicalar Cotton was 2.0 mg.L-1.12, There were different radiation sensitivity with different genotype varieties. The sensitivity of Lilium davidii var. unicalar Cotton was better than Lilium longiflorum.13 , The effects of different concentrations and time of colchicines liquid treatment with different genotype varieties. Best treatment for inducing frequency was obtained when the concentration of colchicines was 0.7% for 5 days, the inducting frequency of Lilium davidii var. unicalar Cotton being 40%, and Lilium longiflorum being 30%.14, The effects of mutagenesis on different genotype varieties combined with different colchicines liquid treatment. Lilium davidii var. unicalar Cotton was suit of soaking with colchicines, and Lilium longiflorum was suit of embedding with absorbent cotton.15, Comparative observations on leaf stoma’s numbers of guard cell and size between tetraploid and diploid of Lily showed significant difference.16 , Morphological observation showed that the chromosome numbers of diploid Lilium davidii var. unicalar Cotton and Lilium longiflorum was 24(2n=2X=24,X=12), while that of tetraploid plants was 48(4n=4X=48,X=24).更多还原