【作者】 刚宏林；

【导师】 赵文静；

【作者基本信息】 黑龙江中医药大学 ， 中药学， 2004， 硕士

【摘要】 虎斑游蛇Natrix tigrina Lateralis(Berthold)，又名野鸡脖子，它是一种有着重要药用价值的动物类药材，为游蛇属无毒蛇。虎斑游蛇具有祛风湿，止痹痛，解毒等功能，因为其治疗风湿性关节炎、骨结核等疾病疗效显著，因此在民间被广泛应用。但对其抗炎作用及作用机理方面的系统研究尚未见报道。本实验通过建立多种实验性炎症的动物模型，观察虎斑游蛇的乙醇提取物对急性、慢性炎症的作用，并对其抗炎作用机理进行探讨，从而为临床开发用药和进一步研究提供科学的实验依据。现对本实验的研究方法和结果简述如下： 1．抗炎实验研究 通过虎斑游蛇对蛋清致大鼠足跖肿胀、二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性增高和大鼠棉球肉芽肿影响的实验研究，揭示虎斑游蛇的乙醇提取物对蛋清致大鼠足肿胀、二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性增高及大鼠棉球肉芽肿均有抑制作用，与空白对照组比较，均有显著性差异，且呈剂量依赖性。 2．抗炎机理研究 通过虎斑游蛇对去肾上腺大鼠蛋清性足跖肿胀、对大鼠胸腺重量和对小鼠肝糖原含量及对小鼠嗜酸性粒细胞数量影响的实验研究，表明虎斑游蛇能够抑制去肾上腺大鼠蛋清性足跖肿胀，使小鼠胸腺的重量减轻，增加小鼠肝糖原的含量而减少嗜酸性粒细胞的数量，提示虎斑游蛇具有肾上腺皮质激素样作用。又通过对大鼠肾上腺内维生素C含量及血浆皮质醇含量测定的实验研究，结果发现虎斑游蛇醇提物并不降低肾上腺内维生素C含量，只显现出降低肾上腺内维生素C含量的趋势，反而降低大鼠血浆皮质黑龙江中医药大学硕士学位论文中文摘要醇含量，显示出虎斑游蛇对大鼠肾土腺皮质激素的合成和释放无作用，其抗炎作用与下丘脑一垂体一肾上腺皮质轴的关系不大，其抗炎作用与皮质轴关系需有待于进一步深入研究。3.对炎症介质的作用 通过虎斑游蛇对大鼠炎性渗出物‘}，组胺和5一挂色胺含量的测 声定，发现其能够减少渗出液中组胺和5一烃色胺的含量，表明虎斑游蛇能够抑制炎症介质组胺和5一经色胺的释放，而发挥抗炎作用。 综上所述，通过对虎斑游蛇抗炎作用及其机理的实验研究，揭示了其具有肾上腺皮质激素样作用的特点，同时能够抑制炎症介质的释放，而发挥抗炎作用，但与下丘脑一垂体一肾上腺皮质系统的作用无关。 通过上述实验研究，基本全面评价和解释了虎斑游蛇在抗炎方面的作用特点及作用机理，亦初步验证了虎斑游蛇祛风湿，止痹痛，治疗风湿病的传统功用，为合理开发和药川虎斑游蛇资源提供了坚实的科学依据。更多还原

【Abstract】 Natrix tigrina Lateralis (NTL) is also named neck-pheasant. It is one of the zonic medicinal materials with important medicinal value, and it is nontoxic snake belonging to Colubridae. NTL has the function of expelling wind, eliminating dampness, relieving pain due to rheumatism, detoxification, etc., and because of its remarkable curative effect to rheumatoid arthritis and bone tuberculosis, and so forth, it has been applied far and wide in Chinese traditional folk. But there is no report on the systematic study of anti-inflammation action and mechanism of anti-inflammation. In our experiment, we observe the curative effect of NTL ethanol extract to acute and chronic inflammation in rats and mice by using several experimental study models, and its mechanism of anti-inflammation is discussed. The purpose is to provide a scientific experimental basis for clinical development and further research. Method and results are as follows:1.The experimental study of inflammationThis experiment opens out that NTL ethanol extract can inhibit the edema of rat hind paw induced by fresh egg white, the ear-swelling of mice induced by xylene, the celiac capillary of mice induced by acetic acid and the proliferation of grannuloma of rat induced by cotton ball through experimental study of effect of NTL on edema of rat hind paw induced by fresh egg white, ear-swelling of mice induced by xylene, celiac capillary of mice induced by acetic acid and proliferation of grannuloma of rat induced by cotton ball, and there is significant difference between NTL and control group, in a dose-dependent manner.2.The study of mechanism of its anti-inflammationThis experiment shows that NTL can inhibit the edema of hind paw induced by fresh egg white in adrenalectomized rat, reduce the weight of thymus in rat, increase the content of hepatic glycogen ,but decrease eosinophil of mice through experimentalstudy of effect of NTL on edema of hind paw induced by fresh eggwhite in adrenalectomized rat, weight of thymus in rat, content ofhepatic glycogen and eosinophil of mice. The results indicate that NTL has the analogous function to cortin. In the experimental study on the content of Vitamin C in adrenal gland and plasma hydrocortisone, the result shows NTL can’t decrease the content of Vitamin C in adrenal gland, just appear the trend of decreasing the content of Vitamin C in adrenal gland, on the contrary, it can decrease the content of plasma cortisol in rat, which shows it has no effect to the synthesis and liberation of rat’s adrenal cortical hormone.and its anti-inflammation function hasn’t much relationship to hypothalamus-pituitary-adrenal axis, so the relationship between the anti-inflammation and cortex needs further research.3. The effect to inflammatory mediatorsIn the assay of inflammatory exudates and 5-HT content, it can decrease the content of histamine and 5-HT in exudates, which shows that NTL effects anti-inflammation through inhibiting the liberation of inflammatory mediators histamine and 5-HT.To sum up, the experimental study on the anti-inflammation function and its mechanism of NTL shows it has the analogous function to the adrenal cortical hormone, and can effect anti-inflammation function though inhibiting the liberation of inflammatory mediators, but this function has no relationship tohypothalamus-pituitary-adrenal axis.Through the experimental study above, the anti-inflammation characteristic and mechanism of NTL is given basically overall evaluation and explanation, and its traditional function of expelling wind, eliminating dampness, relieving pain due to rheumatism andtreating rheumatism is tested elementarily, which provides stablescientific basis for reasonable exploiture and use of NTL.更多还原