【作者】 霍光；

【导师】 钟德钰；

【作者基本信息】 青岛大学 ， 口腔临床医学， 2004， 硕士

【摘要】 目的：探讨几丁质及其衍生物几丁聚糖制成的壳聚糖-PVA-羧甲基壳聚糖膜(C-CM膜)的组织相容性，可降解性，促进创面愈合，止血及抑菌作用，以便对C-CM膜用于牙周引导组织再生的可行性进行初步的生物学评价。 方法：64只大鼠分成3组。第一组42只，3种不同厚度的膜植入大鼠腿部肌肉与植入外科缝合丝线组作对照，按照1周和2，3，4，5，6周时间点取标本做组织病理学检查，考察其体内降解性及其组织相容性；第二组12只大鼠，在每只大鼠背部两侧制备全厚皮片缺损促进愈合的动物模型，4个1.0×1.0cm创面，一侧敷以C-CM膜，另一侧裸露作空白对照，测量伤口愈合面积；第三组10只大鼠，制备大鼠全厚皮片缺损止血的动物模型，在每只大鼠背部两侧做4个1.0×1.0cm创面，随机分别用C-CM膜和纱布止血，观察与创面的黏附情况，记录出血时间，45min后，取下C-CM膜和纱布，并用紫外分光光度仪测膜或纱布中血红蛋白光度吸收值表示出血量，测量其止血效果；采用固体培养基体外抑菌法，观察C-CM膜对细菌(金黄色葡萄球菌ATCC 27583、大肠杆菌ATCC25922、白色念珠菌ATCC 14053)生长的影响。 结果：C-CM膜植入动物肌肉中，周围组织无明显炎症，未发现组织变性和坏死，可在组织内缓慢降解，具有良好的组织相容性，6周后反应接近外科缝合丝线组；止血实验结果发现：C-CM膜组和纱布组的出血时间分别为83.5±9.333s和117±15.9275s，血红蛋白光度吸收值为0.9204±0.7559和1.9764±0.7205，二组比较差异显著(P＜0.05)，说明C-CM膜有一定的止血作用；促进创面愈合实验结果显示：不同时间段C-CM膜组肉芽组织较对照组厚，其中胶原纤维含量亦较多，C-CM膜组的创面愈合时间早于空白对照组，伤口愈合较快；抑菌实验显示C-CM膜对三种菌产生接触性抑制。 结论：C-CM膜有良好的生物学性能，能满足作为牙周引导组织再生膜的基本要求，有可能成为一种较理想的牙周引导组织再生的生物膜材料。更多还原

【Abstract】 Objective: The objective of the study was to explore the biocompatibility,biodegradability ,hemostatic and biological effects of chitosan-PVA-cm-chitosan membranes(C-CM), for the possibility of using C-CM membrane in guided tissue regeneration.Methods: 64 Wistar rats were divided into 3 groups. In the first group, the 3different thick C-CM membranes were implanted in 42 rats leg muscular porch .The specimen were examined histologically 1 week and 2. 3. 4. 5. 6 weeks respectively ;In the second group , full thicle flap wounds were made in 12 Wistar rats bilaterally. One wound in each animal was redressed by C-CM fiim ,the other was exposed as the control. The healing process was studied by the changes of depth and width of wounds. The third group included 10 Wistar rats . Four full thick flap wounds were made on the back of the rats. Using a randomized design, two wounds in each animal was treated with C-CM film, the others were treated with gauze. Bleeding times were measured for the bilateral back wounds. After bleeding sloped, the hemoglobin amount in films or gauzes were measured to determine the hemostatic effect Using a randomized design, one incision in each animal was treated with C-CM membrane, the influence of C-CM film on the growth of bacteria (Staphylococcus aureus ATCC 27853. Escherichia coli ATCC 25922. Monillia albicans ATCC 14053)were observed in vitro with solid medium.Rusults: It was observed that C-CM membrane had no toxic effects onsurrounding tissue and was slowly degraded after implanted into rat leg muscular porch ; The results of hemostatic experiment showed that C-CM film adhered well to incisions. Bleeding time and hemoglobin amouts in C-CM film group were less than those in gauze group (P<0.05), the content of granulation tissue and collagen formation in the C-CM films redressed wounds was moreshort than the control; The results showed the C-CM membrane touched inhibition on the three kinds of tested bacteria .Conclusion: The C-CM membrane possesses satisfactory properties invivo,and the C-CM membrane could be therefore applied as a biological material for GTR.更多还原