【作者】 魏燕芳；

【导师】 陈盛；

【作者基本信息】 福建师范大学 ， 高分子化学与物理， 2004， 硕士

【摘要】 本文报道三方面的内容：(1)对耐酚热带假丝酵母Candida tropicalis(简写为Ct)的优化发酵条件进行研究，并用正交实验法确定了其除酚发酵的最适培养基。(2)制备用于固定Ct的载体，用反相悬浮聚合法合成磁性壳聚糖微球，再进行化学改性，引入胺基，使其碱性增强，成为新型的磁性壳聚糖胺基衍生物，并用元素分析法对其进行了表征。然后，分别以磁性壳聚糖微球和胺基化磁性壳聚糖微球为载体固定Ct。结果表明：以改性后的胺基化磁性壳聚糖微球为载体固定Ct的理化性质优于磁性壳聚糖微球。(3)初步探讨了胺基化磁性壳聚糖微球固定Ct对模拟含酚废水的催化氧化效率和对实际废水的处理情况。结果表明：在模拟含酚废水处理中，胺基化磁性壳聚糖微球固定Ct的反应活性比磁性壳聚糖微球固定Ct高；同时将其用于福建泉港炼油厂的含酚废水处理，也取得了很好的效果。更多还原

【Abstract】 This paper reports three aspects as follow: First, the optimumcomposition of a fermentative medium for phenol-resisting Candida tropicalis was decided by the orthogonal test. Second, a new carrier to immobilized Candida tropicalis was synthesized. A novel aminated magnetic chitosan microspheres were prepared from chitosan flake through inverse suspension cross-linking in the presence of magnetic particles, then hydroxylpropyl chloridization and amination. Besides, it was characterized by Element Ultimate Analysis. Then, as a carrier, the immobilization of Candida tropicalis on aminated magnetic chitosan microspheres and magnetic chitosan microspheres were studied. The results showed that aminated magnetic chitosan microspheres immobilizing Candida tropicalis had the better physicochemical properties than magnetic chitosan microspheres immobilizing Candida tropicalis. Third, probed into the catalytic oxidizing efficiency of aminated magnetic chitosan micropheres immobilizing Candida tropicalis used to deal with man-made and factory wastewater. With regard to man-made wastewater, the results showed that the reaction activity of aminated magnetic chitosan microspheres immobilizing Candida tropicalis was superior to magnetic chitosan micropheres immobilizing Candida tropicalis. At the same time , we also gained a good effect as to factory wastewater which from oil refinery in QuanGang area of Fujian province.更多还原