【作者】 姜代勋；

【导师】 黄会岭；

【作者基本信息】 河北农业大学 ， 临床兽医学， 2004， 硕士

【摘要】 本项目探讨小尾寒羊附红细胞体病的发病机制，并研究羊附红细胞体病中药治疗的新方法。试验动物分为对照组和实验组，实验组又分为阳性对照组和治疗组，每组10只羊。治疗组共4组，分别为头翁四君子汤组、青蒿四君子汤组、丹皮生地散组（中药对照组）及土霉素组（西药对照组）。对照组、实验组在用药前1d，用药后第1d、第4d和第7d分别自颈静脉和耳静脉采血，检测红细胞C_3b受体花环率(C_3bRR)、免疫复合物花环率（ICR），外周血T淋巴细胞转化值及血清一氧化氮（NO）、超氧化物歧化酶（SOD）、丙二醛（MDA）、血清总蛋白（TP）、白蛋白（Alb）等指标，并观察记录每只羊的精神、食欲等临床症状及测量体温。结果表明实验组不但在临床症状上与对照组存在差异，而且在免疫、生化指标上有显著差别，表现在红细胞免疫功能降低，外周血T淋巴细胞转化值升高，血清NO含量异常升高，SOD活力下降，MDA含量升高等。治疗组用药后，头翁四君子汤组红细胞免疫功能明显增强，外周血T淋巴细胞值显著升高，血清NO明显下降，SOD活性增强，MDA含量降低等，且临床症状明显减轻，精神好转，食欲增强，体温明显下降。青蒿四君子汤组红细胞免疫功能明显增强，外周血T淋巴细胞值变化不明显，血清NO下降，SOD活性变化不显著，MDA含量显著降低等，临床症状改善，精神状况好转，食欲提高，体温下降。丹皮生地散组红细胞免疫功能、外周血T淋巴细胞值、血清NO变化不显著，SOD活力明显增强，MDA含量降低等，临床症状有所缓解，食欲增加。土霉素组红细胞免疫功能变化不明显，外周血T淋巴细胞值明显升高，血清NO在用药后先明显下降继而持续增高并超过用药前水平，SOD活力变化不明显，MDA用药后先急剧升高，第4d明显下降等，临床症状有所减轻，精神状况、食欲稍有改善。用药后，治疗组红细胞附红细胞体感染率均显著下降，但以头翁四君子汤组和青蒿四君子汤组下降程度最大，明显低于丹皮生地散组和土霉素组。结果表明：患附红细胞体病羊红细胞免疫功能显著降低，外周血T淋巴细胞转化值升高，血清NO含量异常升高，SOD活性增强，MDA含量升高等；治疗组用药后，以头翁四君子汤疗效最好，各血液指标基本恢复正常，临床症状明显减轻，其次是青蒿四君子汤，明显优于丹皮生地散和土霉素。更多还原

【Abstract】 The aim of this project was to investigate the pathogenesis of eperythrozoonosis in Han sheep(small tail). Han sheep (small tail) were divided into normal control group and experiment groups, experiment groups were divided into positive control group and treatment groups, treatment groups included Touweng Sijunzi Tang group, Qinghao Sijunzi Tang group, Danpi Shengdi Tang group and terramycin group, every group had ten sheep, normal control group and experiment groups collected blood from jugular vein and scapha vein respectively the previous day of treatment and the 1st day, the 4th day and the 7th day after treatment, totally 4 times, examining erythrocyte immune function, T lymphoid cell value of peripheral blood and serum NO, SOD, MDA, TP, Alb, etc. and observing, recording the clinical symptoms and the general appearance, appetite condition, temperature of each sheep. The results of the experiment indicated that experiment group was different from normal control group not only in clinical symptoms, but also in blood parameters, such as descent of erythrocyte immune function and ascent of T-lymphoid cell value of peripheral blood, unusually high serum NO content, descent of activity of serum SOD, obvious ascent of MDA content, etc. After treatment, in Touweng Sijunzi Tang group, erythrocyte immune function rised, T lymphoid cell value of peripheral blood dramatically ascended, serum NO dramatically reduced, activity of SOD rised, MDA content descended, etc. and clinical symptoms obviously improved, the general appearance rised, the appetite enhanced, temperature descended dramatically. In Qinghao Sijunzi Tang group, erythrocyte immune function dramatically improved, T lymphoid cell value of peripheral blood changed unclearly, serum NO descended, activity of SOD came to decrease, MDA content dramatically descended, etc. clinical symptoms were ameliorative, the general appearance and the appetite condition builded up, the temperature reduced. In Danpi Shengdi Tang group, except that activity of SOD markedly increased, MDA content prominently descended,the remaining parameters didn’t change remarkably. Clinical symptoms improved only a little, the appetite enhanced. In terramycin group, erythrocyte immune function had no distinct change, T lymphoid cell value of peripheral blood markedly ascended, serum NO dramatically descended at first and ascended at 4th day and 7th day, activity of SOD had no distinct change, serum MDA dramatically ascended at first after treatment and dramatically descended in the 4th day, Clinical symptoms improved litter, the general appearance and the appetite were not ameliorative. After treatment, erythrocyte infection rate of eperythrozoon of the four treatment groups dramatically descended, but the descent extent of Touweng Sijunzi Tang group and Qinghao Sijunzi Tang group were greater than that of Danpi Shengdi Tang group and terramycin group. The results indicate that in affected eperythrozoonosis sheep erythrocyte immune function dramatically descend, T lymphoid cell value of peripheral blood rises, serum NO of eperythrozoonosis sheep unusually ascends, SOD activity descends, serum MDA content ascends, etc. After the therapy of treatment groups, curative effect of Touweng Sijunzi Tang was the best, it made immune parameters recover, clinical symptoms prominently improved. The second best was Qinghao Sijunzi Tang, superior to Danpi Shengdi Tang and terramycin.更多还原