【作者】 田金强；

【导师】 张子德； 马俊莲；

【作者基本信息】 河北农业大学 ， 农产品加工及贮藏工程， 2004， 硕士

【摘要】 近年来，红提葡萄的生产在我国发展迅速。红提葡萄产区和成熟期较集中，常温下易腐烂，上市时间短，典型的鲜食品种而不适宜大规模的加工，因此，红提葡萄生产的持续、健康、稳定的发展必须以贮藏保鲜业作为后盾。然而，红提葡萄的贮藏保鲜技术仍然没有完全解决。突出的问题是红提葡萄贮藏保鲜过程中极易发生SO₂伤害，严重影响了果实的商品价值和食用价值。本试验主要研究了防止红提葡萄贮藏保鲜过程中发生SO₂伤害的方法，同时，对可取代SO₂的保鲜剂和保鲜方法做了初步研究。主要研究结果如下： 1．导致红提葡萄腐烂的主要病原菌为交链孢霉（Alternaria SPP.）。其次为灰葡萄孢霉（Botrytis cinerea）。 2．对于灰葡萄孢霉，过氧乙酸、双氧水、对羟基苯甲酸乙酯的抑制能力最强，其次为次氯酸钠、冰醋酸，新洁尔灭、蜂胶、扑海因和次亚磷酸钠的抑制能力相对较弱，以上药剂的最小抑制浓度（MIC）依次为0.02％、0.02％、0.04％、0.08％、0.1％、0.4％、0.5％、0.16％、1.0％；对于交链孢霉，扑海因、新洁尔灭、对羟基苯甲酸乙酯抑制能力最强，其次为过氧乙酸、双氧水、次氯酸钠和冰醋酸，蜂胶和次亚磷酸钠抑制能力相对较弱，以上药剂的最小抑制浓度（MIC）依次为0.005％、0.025％、0.04％、0.02％、0.02％、0.08％、0.1％、0.5％、1.0％；其它药剂如苯甲酸钠、山梨酸钾、蔗糖脂肪酸酯、壳聚糖等对两种霉均没有抑制效果。 3．用正常剂量的SO₂（7袋CT₂／5Kg果穗）配合2％多聚磷酸钠、2％酪氨酸钙、2％KI和0.5％I₂混合物、0.5％KI、I₂（1g／5Kg果穗），和低于正常剂量的SO₂（3袋CT₂／5Kg果穗）配合0.4％新洁尔灭、0.4％对羟基苯甲酸乙脂、包埋的0.4％对羟基苯甲酸乙脂、0.2％对羟基苯甲酸乙脂和0.2％新洁尔灭混合药剂、包埋的0.2％对羟基苯甲酸乙脂和0.2％新洁尔灭混合药剂、0.4％对羟基苯甲酸乙脂和1％BHT混合药剂、0.4％对羟基苯甲酸乙脂和2％KI混合药剂、1％蜂胶、1％蜂胶和0.2％植酸混合药剂处理红提葡萄，均可有效防止红提葡萄在MA条件下贮藏的SO₂伤害。但以上处理的防腐效果和防褐变效果不同，和单独使用正常剂量的SO₂相比较：SO₂配合I₂，优于其（正常剂量的SO₂，下同）防腐效果，但不能达到其防褐变效果；SO₂配合KI，接近其防腐效果，优于其防褐变效果；SO₂配合酪氨酸钙、多聚磷酸钠、KI与I₂混合物，接近其防腐效果，但不能达到其防褐变效果；SO₂配合包埋的对羟基苯甲酸乙脂和新洁尔灭，优于其防腐效果，接近其防褐变效果；SO₂配合蜂胶或蜂胶和植酸的混合药剂，接近其防腐效果，但不能达到其防褐变效果；其它药剂和SO₂配合，不能达到其防腐效果和防褐变效果。防止SO₂伤害和腐烂的最佳处理均为：低于正常剂量的SO₂配合包埋的对羟基苯甲酸乙脂和新洁尔灭。防止褐变的最佳处理为：正常剂量的SO₂配合KI。 4．适宜浓度的SO₂（3袋CT₂／5Kg果穗）和O₃配合使用，无论是每d施加一定量O₃（浓度2μl／l、4μl／l），还是每7d施加一定量O₃（浓度为8 μl／l），均能有效防止红提葡萄在CA贮藏中的SO₂伤害，并具有显著优于正常剂量SO₂（7袋CT₂／5Kg果穗）的防腐效果。但防褐变效果根据O₃的剂量而定：2μl／l的O₃每d处理一次，或8μl／l的03每7d一次，均优于正常剂量502的防喝变效果:扭1/l的O:每d处理一次，不能达到正常剂量502的防褐变效果。5.适宜浓度的502和03配合使用，能抑制果实的还原糖、可滴定酸和VC的消耗，抑制果实MDA的产生，延缓果实的成熟和衰老。其效果优于单独使用50:类保鲜剂，更优于单独使用。3。6.药剂的包埋处理可显著提高药剂的防腐、保鲜能力。7.班具有优异的防褐变效果。BHT和植酸也具有一定的防褐变效果。8.单独使用03不能长期贮藏红提葡萄。幸1/l一1伽1/1的。3浓度每d处理一次或助l/l的03浓度每7d处理一次，果粒、穗轴和果梗均发生严重的褐变、腐烂现象。9.MA条件下，防止红提葡萄502伤害的最佳措施为:采前喷撒经包埋处理的0.2%对轻基苯甲酸乙脂和0.2%新洁尔灭混合药剂，入贮时在包装袋内放入低剂量的CTZ502类保鲜剂（4包/5Kg果穗）。在贮藏温度为一1℃、相对湿度为90%一95%条件下贮藏180d，果实的各项指标为:腐烂率3.09%、漂白指数1.98%、穗轴褐变指数14.84%、果梗褐变指数8*37%、还原糖含量14.巧%、可滴定酸含量0.42%、Vc含量0.52m留1009和50:残留量1.09m乡吸g。10.CA条件下，防止红提葡萄502伤害的最佳措施为:贮藏环境内保持一定浓度的502，如CTZ（3包/5Kg果穗），每d充入一定剂量的O:（取l/l）。在贮藏温度为一1℃、相对湿度为90%一95%、。23%⁵%、COZ(5%条件下贮藏200d，果实的各项指标为:腐烂率5.00%、漂白指数2.18%、穗轴褐变指数9.09%、果梗褐变指数10.07%、还原糖含量10.09%、可滴定酸含量0.34%、VC含量o.68mg/ioog和50:残留量1.64m妙g。更多还原

【Abstract】 In the recent years, the production of Red Globe table grape has been progressing rapidly. Red Globe table grape is a kind of fruit with relatively concentrative producing areas and harvesting time; it is perishable on normal temperature, and its marketable time is rather; short. .Moreover, being a typical table variety, the massive process is fairly difficult. Therefore, the storage and .fresh-keeping industry is vital to the continuous, healthyy and steady development of Red Globe grape’s production. However, this technology has not been solved perfectly, in which the most outstanding problem is that grapes under storage are easy to incur SO2 Injure, which has a serious effect on their edible value and commercial value. The SO2 Injure preventing techniques were studied. In addition, some alternative fresh-keeping agents and methods were studied as well. Results showed:1. The leading pathogeny microbes resulted in decay was Alternariaspp, followed by Botrytis cinerea.2. To Botrytis cinerea, the most potent antibacterial agents were Peracetic acid, Hydrogen peroxide and Ethyl p-hydroxybenzoate, followed by Sodium hypochlorite and Acetic acid, Bromo-Geramium, Propolis, Iprodione and Sodium hypophosphite were relatively weak. The Minimum Inhibitory Concentrations (MIC) of the agents listed above were 0.02%, 0.02%, 0.04%, 0.08%, 0.1%, 0.4%, 0.5%, 0.4%, 0.16% and 1.0% respectively. As to Alternaria spp., Iprodione, Bromo-Geramium and Ethyl p-hydroxybenzoate had the most effective prohibiting power, witii Peracetic acid, Hydrogen peroxide, Sodium hypochlorite and Acetic acid in the secondary rank, Propolis and Sodium hypophosphite in the bottom of the hierarchy. The MIC for all of these agents could be 0.005%, 0.025%, 0.04%, 0.02%, 0.02%, 0.08%, 0.1%, 0.5% and 1.0% respectively. Nevertheless, other preservatives used commonly in food, such as Sodium benzoate, Sorbate, Sucrose Fatty Acid Ester and Chitosan, were totally ineffective to the two moulds.3. Using SO2 with the normal dosage (7bags CTi/SKg grape fruits), mixed with 2%Sodium polyphosphate, 2%Calcium caseinate, 0.5%Potassium iodide, Iodine (1g/5kg grape fruit )or mixed agents including 2%Potassium iodide and 0.5%odine, could prevent SO2 Injure effectively. And Using SO2 with dosage less than normal(3bags CT2/5kg grapefruit), mixed with 0.4%Bromo-Geramium, 0.4%Ethyl p-hydroxybenzoate, 0.4% Ethyl p-hydroxybenzoate microencapsulated, the mixed agents microencapsulated including 0.2%Bromo-Geramium and 0.2%Ethyl p-hydroxybenzoate, the mixed agents including 0.2%Bromo-Geramium and 0.2%Ethyl p-hydroxybenzoate, 1% Propolis or the mixed agents including 1% Propolis and 0.2% Phytic acid could prevent SOj Injure effectively as well.However, using SOa of different dosages mixed other agents, these treatments were different in inhibiting decay and browning comparing with just use 862 of the normal dosage: mixed with Iodine, had better inhibition effect on decay, but had worse effects on browning; mixed with Potassium iodide, had better inhibition effect on browning while the effects on decay are not so good; mixed with agents such as Calcium casemate,. Sodium polyphosphate, mixed agents including Potassium iodide and Iodine, had the same good effects on decay, but effects on browning would be worse; mixed with the mixed agents microencapsulated, iscluding Bromo-Gcramium and Ethyl p-hydroxybenzoate, had better inhibition effects on decay and the same browning inhibiting effects; mixed with Propolis or the mixed agents including Propolis and Phytic acid, had the same effects on decay, but didn’t have so good effects in browning; mixed with the other agents, couldn’t be so good in inhibiting browning and decay. Using SO2 with dosage less than normal (3bags CT2/5Kg grape fruit), mixed with the mixed agents microencapsulated, including Bromo-Geramium and Ethyl p-hydroxybenzoate, was the best treatment to prevent SO2 Injure or decay. SO2 with normal dosage, mixed with Potassium iodide, was the best treatment to prevent browning.4. Integrated with O3, no matter it’s a更多还原