【作者】 洪梅；

【导师】 黄璜； 梁红；

【作者基本信息】 湖南农业大学 ， 作物栽培学与耕作学， 2003， 硕士

【摘要】 研究表明，银杏黄酮对水稻幼苗的伸长生长有轻微的抑制作用，有壮苗作用，能促进水稻幼苗鲜重的增加，其SOD、CAT和POD活性也与对照有差异。银杏黄酮类化合物包括槲皮素等苷元及其衍生物，共约40余种，有清除自由基的功能，对自由基引起的细胞损伤和染色体畸变有较强的保护和修复作用。 本实验通过银杏黄酮的主要苷元槲皮素对绿豆幼苗的处理发现，用不同浓度的槲皮素对绿豆幼苗进行处理，在黑暗条件下槲皮素对绿豆幼苗下胚轴的伸长有轻微抑制作用，但有壮苗作用，能促进幼苗下胚轴增粗和鲜重增加，且这种作用伴随槲皮素浓度的增加逾加明显。对绿豆幼苗3种保护酶的测定表明，经槲皮素处理后的绿豆幼苗，其SOD下降，CAT上升，而POD活性则在低浓度表现为下降，在高浓度范围表现为上升。在光照条件下，槲皮素处理促进绿豆幼苗的苗高、鲜重及单位长度苗重的增加。 在对槲皮素耐辐射的研究中发现，槲皮素处理的绿豆幼苗经5Gy和9Gyγ射线辐射后，其光合速率明显高于对照；电导率在辐射当天表现升高，24h后恢复至对照(水溶液处理)水平，未经槲皮素处理的绿豆幼苗的电导率一直处于较高水平；而SOD活性在在低剂量表现为低-高-低，在高剂量表现为升高。P O D、CAT活性则与辐射时间长短和剂量有关，低剂量处理在辐射第一、二天表现为升高，第三天呈现下降。高剂量处理者均高于对照。 本研究初步肯定了槲皮素对植物有壮苗作用，能促进胚轴增粗增重；对绿豆幼苗的辐射损伤有一定的保护作用；并就槲皮素对植物生长的作用和可能的应用前景进行了讨论。更多还原

【Abstract】 Research indicated that Quercetin could inhibit elongation and promote diameter and fresh weight increasing of the rice seedlings, and its SOD, CAT and POD activity was different from the control. Flavonoid including Quercetin,keampferol,iso-Rhamnetin, and their derivatives,more than 40 kinds, could scavenge free radical, protect or repair cell from damage and aberrance of chromosome.In this paper,the research results showed that Quercetin in different concentration could inhibit elongation of mung bean seedling in dark, but strengthen the seedling by promoting its diameter and fresh weight. The effect was more evident with the higher content of Quercetin used. The activity of SOD decreased, but the activity of CAT increased, while the activity of POD decreased with lower content of Quercetin treatment,increased in higher content of Quercetin treatment.Under lighting, the Quercetin treatment increased mung bean seedling’s height, the fresh weight and the fresh weight per centimeter of the mung bean seedling.In the study of Quercetin’s radiation resistance , we also discovered that after radiation,the Photosynthesis rate of mung bean seedling treated by Quercetion was higher than the control, the conductivity increased but recovered to the level of the control after 24 hours. Without Quercetin treatment, the conductivity of mung bean seedlings treated by radiation was keeping higher level, and the change of SOD activity represented low-high-low in lower content of Quercetin treatment,and increased in higher content of Quercetin treatment. The activity of POD and CAT were related to the length of time and the dose of radiation, increasing in the first day and the second day, decreasing in the third day, but it was still higher than the control in higher content.Study result infirmed that Quercetin could inhibit mung bean seedling’s elongation, promot diameter and fresh weight,and protect mung bean seedling from damage by radiation.更多还原