【作者】 曾明；

【导师】 郭层城；

【作者基本信息】 西北师范大学 ， 体育教育训练学， 2004， 硕士

【摘要】 众多研究报告，运动会引起体内自由基的大量生成，进而攻击机体生物膜系统，导致细胞结构破坏和功能紊乱。松针提取液是一种高效无毒的天然抗氧化剂，它富含维生素E、维生素C、β-胡萝卜素及黄酮类化合物前花青素等天然抗氧化剂。本实验目的在于揭示松针提取液对运动小鼠的自由基防御体系、运动能力和糖代谢的影响，为松针提取液在运动医学领域应用和运动保健饮料、食品的开发提供实验依据。 实验以昆明种小鼠(雌雄各半)为实验对象，以递增负荷游泳训练方式建立耐力性运动模型。测定心肌组织、肝组织和股四头肌中SOD、MDA、T-AOC，血清LA、LDH、BUN，肝组织和股四头肌糖原。 实验结果表明：(1)大强度耐力训练后，服用松针提取液小鼠的Hb含量有所升高，说明松针提取液可以改善大强度耐力训练后Hb含量降低的现象，使小鼠Hb含量明显上升，提高有氧运动能力。(2)服用松针提取液的小鼠血尿素氮含量明显低于其对照组，说明松针提取液可以降低运动小鼠体内蛋白质分解代谢速率，保持肌力，延缓运动性疲劳的发生。(3)松针提取液可以增加小鼠体内糖储备，保证中枢神经系统、运动肌肉等组织的能量供给，延缓运动性疲劳的发生，提高运动能力。(4)松针提取液对小鼠心脏组织、肝脏组织、肌肉组织都表现出明显的抗脂质过氧化和清除自由基的能力。同时表现出对同一状态下不同组织内同一抗氧化酶活性的影响不同，对同一抗氧化酶在同一组织内不同状态下活性的影响也不同。 结论：松针提取液具有很强的抗脂质过氧化、清除自由基的作用，它可以改善耐力训练对机体产生的某些不良影响，从而提高运动能力。更多还原

【Abstract】 Many studies show that exercise can cause body to produce lots of free radicals that will attack cellular membrane system and result in the damage of structure and the malfunction of cells. The extract of pine needles is a natural antioxidant that is efficient and non-poisonous. It contains Ve, Vc, a carotene, and many natural antioxidiants such as PCA, a flavone compound. The purpose of this experiment is to discover the effect of the extract of pine needles on swimming rats’ free radical defense system, exercise performance, and carbohydrate metabolism, and to provide experimental basis for the utilization of the extract of pine needles in sports medicine and the development of health drinks and food.Objects of the study are Kunming rats (half male and female) and an endurance exercise model is set up by progressive load swimming training. SOD, MDA, T-AOC in cardiac muscular tissue, hepatic tissue, and musculus quadriceps femoris are tested; LA, LDH, BUN of serum and glycogen in hepatic tissue and musculus quadriceps femoris are tested.Results of the experiment: (1) After intensive endurance training, Hb of rats taking the extract of pine needles is increased, which indicates that extract of pine needles can promote the decrease of Hb after intensive endurance training and promote aerobic exercise performance. (2) BUN of rats taking the extract of pine needles is obviously less than that of the control group, which shows that the extract of pine needles can slow down rats’ metabolism rate of proteolysis, hold myotome and delay exercise-induced fatigue. (3) The extract of pine needles can increase the carbohydrate reserve of rats, guarantee the supply of energy to central nervous system and muscles, delay exercise-induced fatigue., and promote exercise performance. (4) The extract of pine needles has strong actions on antioxidation and clearing free radical in rats’ cardiac tissue, hepatic tissue, and muscles. It also has different influence on the activity of the same antioxidase in different tissues under same conditions or that in the same tissue under different conditions.Conclusions: The extract of pine needles has strong actions on antioxidation and clearing free radical. It can improve the harmful effects caused by intensive endurance exercise on body and promote the capability of exercise.更多还原