通关胶囊对TURP术中出血和前列腺组织血管形成影响的机理研究

【作者】 谢淑武；

【导师】 张蜀武； 王久源；

【作者基本信息】 成都中医药大学 ， 中西医结合临床， 2004， 硕士

【摘要】 目的：评价通关胶囊对良性前列腺增生(BPH)患者行经尿道前列腺电切术(TURP)术中出血的影响，并从血管形成的角度探讨通关胶囊减少TURP术中出血的机理。 方法：选择具备手术指征的BPH病人39例，应用1∶1∶1随机数字表，按入院顺序随机分为通关胶囊组、非那雄胺组和安慰剂组。服药2周后，行TURP术，计算术中出血量、切除每克腺体出血量和每分钟出血量。用免疫组化法结合图像分析系统研究正常组、安慰剂组、非那雄胺组和通关胶囊组前列腺组织AC133、KDR、HIF-1的阳性表达。 结果：通关胶囊组和非那雄胺组的术中出血总量、每克腺体出血量、每分钟出血量与安慰剂组相比均明显减少(P＜0.05)；免疫组化结合图象分析系统研究结果表明，安慰剂组前列腺组织的AC133、KDR、HIF-1的阳性表达较正常组显著增强(P＜0.01)；通关胶囊组、非那雄胺组前列腺组织的上述指标的阳性表达则较安慰剂组明显减弱(P＜0.05或P＜0.01)。 结论：BPH与血管形成有关。通关胶囊能减少TURP术中出血，其机理可能与抑制增生前列腺组织AC133、KDR、HIF-1的表达，进而抑制BPH血管形成有关。更多还原

【Abstract】 Objective: To evaluate influence of Tongguan Capsule on bleeding during TURP on BPH patients and study mechanism of it inhibiting bleeding during TURP through the point of vasculogenesis on BPH.Methods: 39 BPH patients, having surgery sign, were randomly divided into 3 groups-Tongguan Capsule group, finasteride group and placebo group with each group having 13 patients according to hospitalizing orders. TURP were operated and general bleeding volume, bleeding volume per gram and bleeding volume per minute were calculated after 2 weeks of administration. Expressions of AC 133 antigen, KDR and HIF-1 were studied in prostatic tissues in all groups (including normal group) by immunohistochemistry and image analysis system.Results: General bleeding volume, bleeding volume per gram and bleeding volume per minute in both Tongguan Capsule group and finasteride group decreased significantly compared with those of placebo group (P<0.05). The results of immunohistochemistry and image analysis system manifested that expressions of AC 133 antigen, KDR and HIF-1 in hyperplastic prostate of placebo group significantly increased compared with those of normal group,at the same time,those of both Tongguan Capsule group and finateride group significantly reduced compared with those of placebo group(P<0.05 or P<0.01).Conclusions: BPH is related to vasculogenesis. Tongguan Capsule can reduce bleeding during TURP. The principle is possible related to inhibiting Expressions of AC 133 antigen, KDR and HIF-1 in BPH and then inhibiting vasculogenesis.更多还原