脉通方对高糖高脂高胆固醇饮食DM大鼠股动脉内皮功能障碍及相关因素的影响

【作者】 王永山；

【导师】 亓鲁光；

【作者基本信息】 成都中医药大学 ， 中医内科学， 2004， 硕士

【摘要】 目的：研究脉通方对引起糖尿病下肢动脉硬化闭塞症相关因素的影响，主要是糖代谢、脂代谢、氧化应激、血管舒缩功能及血小板功能的影响。方法：采用高糖高脂高胆固醇饲料喂养SD大鼠，并用Alloxan腹腔注射诱发糖尿病大鼠模型。随机分为脉通方低、高剂量组，西药对照组、模型对照组，并设正常对照组。通过放射免疫、生化检测、形态学等方法，对照观察各组药物对DM大鼠一般状态、血糖、血清INS、TC、TG、HDL-C、Lp（a）、SOD、MDA、ET、NO、TXB₂、6-Keto-PGF_1α等指标及股动脉组织形态学的影响。结果：DM大鼠存在大部分指标明显异常及股动脉病理改变，脉通方可改善一般状态、纠正糖、脂代谢紊乱，增强细胞抗氧化能力，调整内皮功能失调，改善血小板聚集功能，综合疗效优于西药。结论：脉通方通过多环节、多途径的调节作用，减轻或消除多种因素对血管内皮的损伤作用，从始动环节上干预糖尿病动脉粥样硬化的发生发展，从而起到防治DLASO的作用。更多还原

【Abstract】 Objective: To investigate the effect of Maitongfang on certain related factors which can cause DLASO, mainly on the effect on glycometabolism, lipometabolism, oxidative stress and function of blood platelet. Methods: The SD rats were fed with high-sugar, high-fat and high-cholesterol diet, and then induced by Alloxan intraperitoneally to be diabetic rat models. The rats were randomly divided into five groups, ie. Model control, low dosage of Maitongfang, high dosage of Maitongfang, and Cilostazol group respectively. Normal control group was also established. The effect of different medicines on the following parameters were detected by radioimmunoassay, biochemistry method, Histomorphometry, etc. which were level of insulin, TC, TQHDL-C,Lp (a) ,SOD, MDA, ET, NO, TXB2,6-Keto-PGF1a, tectology of femoral artery, changes in general condition and blood glucose as well. Results: The abnormality of most of the parameters stated above were shown in DM rats, as well as the pathologic changes of femoral artery. While Maitongfang demonstrates the action of improving the general condition, adjusting the metabolism of blood glucose and blood lipid, strengthening the cell’s ability of antioxidation, correcting the dysfunction of endothelium, improving the function of platelet aggregation. The comprehensive curative effect of Maitongfang is superior to that of Cilostazol group. Conclusion: Through the regulating action of many pathways and links which presented as alleviating or eliminating damnification on Tunica intima of artery by above factors, Maitongfang contributes to the prevention and treatment of DLASO by intervening the very first step of DLASO.更多还原