【作者】 付伟金；

【导师】 莫曾南；

【作者基本信息】 广西医科大学 ， 泌尿外科， 2004， 硕士

【摘要】 目的:探讨A型肉毒杆菌毒素(BTX-A)对鼠前列腺增生模型的影响。方法：64只雄性Wistar大鼠，随机分为模型组(52只)、假手术组(12只)。模型组行去势手术1周后，予皮下注射丙酸睾酮4mg/d.kg造模，连续1周。假手术组同期予皮下注射等量橄榄油,连续1周。随机处死4只模型鼠，取出其前列腺，光镜下观察证实造模成功。将其余48只模型鼠随机分为高、中、低剂量组、阴性对照组，每组均为12只，分别予上述各组前列腺内注射15u/0.1ml BTX-A，10u/0.1ml BTX-A、5u/0.1ml BTX-A、0.1ml生理盐水。继续予皮下注射丙酸睾酮4mg/d.kg造模,连续四周；假手术组继续予皮下注射等量橄榄油,连续四周。各组实验鼠分别于注射2周、4周后分2批(每次6只)处死，取出前列腺、膀胱、心脏、肾脏、肝脏，光镜下观察其形态学变化，计算各实验组前列腺重量、前列腺指数(PI)。病理图像分析系统半定量检测各组腺体面积、间质面积。免疫组化检测bax、fas、bcl-2蛋白表达情况。所有数据用SPSS软件包进行分析。结果：与阴性对照组相比，给药2周时高、中、低剂量组前列腺重量、PI、腺体面积、bcl-2表达下降(P<0.05)；bax、fas表达增加(P<0.05)；间质面积相对增加(P<0.05)。与阴性对照组相比，给药4周时高、中剂量组前列腺重量、PI、腺体面积、bcl-2表达下降(P<0.05)，bax、fas表达增加(P<0.05),间质面积相对增加(P<0.05)。低剂量组腺体面积减少(P<0.05)，低剂量组和阴性对照组间质面积相比，差异无统计意义(P>.05)。给药2、4周时假手术组各项指标比较，差异无统计意义(P>0.05)。结论：BTX-A导致增生鼠前列腺腺体体积缩小，有望成为临床治疗前列腺增生的新方法。更多还原

【Abstract】 Abstract Objective: To investigate effects of botulinum toxin A on rat model of benign prostate hypertrophy.Methods: 64 male Wistar rats were divided into sham operation group(n=12) and model group(n=52). After castrated,52 rats of model group were injected subcutaneously testosterone propionate 4mg/kg.d to induce BPH;12 rats of sham operation group were injected subcutaneously the same dose of olive oil .After 1 week, the 4 model rats were randomly killed.The structure of prostates were observed by light microscopy.It proved that rat model was successful.The other 48 model rats were randomly divided into high dose group、medium dose group、low dose group、negative control group.The high、 medium、low dose group received respectively 15u/0.1ml 、10u/0.1ml 、5u/0.1ml BTX-A injected into the prostate；negative control group undergent 0.1ml NS injection into the prostate at the same position. After injections, the high、medium、low dose group、negative control group rats were injected subcutaneously testosterone propionate 4mg/kg.d for four weeks; sham operation group was injected subcutaneously the same dose of olive oil .The partly rats of the groups were killed randomly at 2、4 weeks. The prostates、bladders、hearts、kidneys、livers and hearts were removed.The structural changes of prostates、bladders、hearts、kidneys、livers and hearts were observed by light microscopy.The prostate weigh、prostate index(PI) were calculated.Morphometric changes of glandul areas、interstitial areas were semi-quantified by image analysis system.The expression of bax、fas、bcl-2 protein were analysed by means of immunohistochemical technique.All data were analysed by SPSS.Results:At two weeks,compared with negative control group, prostate weigh、PI、glandular areas、bcl-2 protein of high、medium、low dose group were significantly decreased(P<0.05),the expression of bax、fas protein significantly increased <WP=5>(P<0.05), interstitial areas relatively increased(P<0.05).At four weeks,compard with negative control group,prostate weighs、PI、glandular areas、bcl-2 protein of high、medium dose group were significantly decreased (P<0.05),the expression of bax、fas protein significantly increased(P<0.05), interstitial areas relatively increased(P<0.05),glandular areas of low dose group remarkablely differences(P<0.05).There were insignificantly differences between low group and negative control group in interstitial areas (P>0.05).There was insignificantly differences between each index of two weeks and four weeks in sham operation group.Conclusions: BTX-A can induce glandular atrophy of the rat model of BPH. Applications of BTX-A therapy could be candidated for the new treatment of BPH.更多还原