【作者】 陈晓波；

【导师】 陈坚；

【作者基本信息】 广西医科大学 ， 泌尿外科， 2004， 硕士

【摘要】 目的：探讨PTEN、CDK4蛋白表达与膀胱移行细胞癌的发生、发展的关系。 方法：采用免疫组化S-P法，测定10例正常膀胱粘膜及40例膀胱移行细胞癌标本中的PTEN、CDK4蛋白的表达情况。 结果：1. PTEN蛋白在正常膀胱粘膜和膀胱移行细胞癌组织中的阳性表达率分别为100%、67.50%，差异有显著性（P<0.05）。2. PTEN蛋白在G1、G2、G3中的阳性表达率依次为89.47%、53.85％、37.50％，G1与G2 或/和G3相比较，差异有显著性（P<0.05）；在Tis -T1中PTEN阳性表达率为80.00%，明显高于T2-T4中的46.67%（P<0.05）。PTEN的表达随病理分级、临床分期的增高而明显下降。3. CDK4蛋白在正常膀胱粘膜和膀胱移行细胞癌组织中的阳性表达率分别为0.00%、52.50%，差异有显著性（P<0.05）。4. CDK4蛋白在G1、G2、G3中的阳性表达率依次为36.84%、61.54％、75.00％，组间比较，差异无显著性（P>0.05）；在在Tis -T1中CDK4阳性表达率为40.00%，明显低于T2-T4中的73.33%（P<0.05）。CDK4的表达随与临床分期密切相关。5. PTEN与CDK4相关性分析显示，二者的表达不相关。结论：PTEN蛋白表达缺失及CDK4的过度表达可能在膀胱移行细胞癌的发生、发展中具有重要作用，PTEN、CDK4蛋白可成为反映膀胱移行细胞癌恶性程度及预后估计的参考指标。更多还原

【Abstract】 Objective: To investigate the expression of PTEN, CDK4 protein in bladder transitional cell carcinoma ( BTCC ) and the relation between their expression and carcinogensis, progression and clinical pathological parameters.Methods: A specifically and sensitively immunohistochemical S-P assay was used to detect the expression of PTEN and CDK4 in paraffin-embedded sections of 40 cases of BTCC and 10 normal bladder mucosa.Result: 1. The positive rates of PTEN protein expression were 67.50% in BTCC tissues and 100% in normal bladder mucosa tissues. There was significant difference between them（P<0.05）.2. The positive rates of PTEN protein expression in G1, G2 and G3 were 89.47%, 53.85％, 37.50％，respectively; and the difference was statistically significant between G1 and G2 or G3（P<0.05）.The positive rate of PTEN protein expression in Tis-T1 was significantly higher than that in T2-T4（P<0.05）. The PTEN protein expression deletion rates in BTCC were significantly associated with histological grades and clinical stages. 3. The positive rates of CDK4 protein expression were 52.50% in BTCC tissues and 0.00% in normal bladder mucosa tissues, and the difference was statistically significant between them（P<0.05）. 4. The positive rates of CDK4 protein expression in G1, G2 and G3 were 36.84%, 61.54％,75.00％,respectively; and there wasn’t statistically significant difference among the three groups （P>0.05）.The positive rate of CDK4 protein expression in Tis -T1 was significantly lower than that in T2-T4（P<0.05）. The CDK4 protein overexpression rates in BTCC were significantly associated with clinical stages.Conclusion: PTEN protein expression deletion and CDK4 protein overexpression are closely related to the occurrence and development of BTCC. To detect PTEN and CDK4 protein together is an important method for the evaluation of the malignancy and prognosis of BTCC.更多还原