【作者】 鲁华东；

【导师】 吕长虹；

【作者基本信息】 重庆医科大学 ， 病理学与病理生理学， 2004， 硕士

【摘要】 目的：探讨明胶酶(MMP-2、MMP-9)及其抑制因子(TIMP-2、TIMP-1)在MPNST中的表达特征及其与临床病理及预后的关系。 方法：应用免疫组织化学S-P法，检测58例MPNST中MMP-2、MMP-9及其抑制因子TIMP-2、TIMP-1的表达，并行回顾性随访。 结果：(1)MMP-2在MPNST中阳性表达率为87.9％，表达水平显著高于正常神经及其良性肿瘤中的表达(P<0.05)；其表达程度与肿瘤组织学分级及生存率密切相关(P<0.05)，与病人性别、年龄、肿瘤大小、部位及术后复发率等无明显相关。MMP-9在MPNST中阳性表达率为43.1％，表达水平高于正常神经及其良性肿瘤中的表达，但无显著性差异；其表达程度与病人性别、年龄、肿瘤大小、部位、组织学分级、术后复发率及生存率等均地无明显相关。(2)TIMP-2在MPNST中阳性表达率为62.1％，表达水平显著低于正常神经及其良性肿瘤中的表达(P<0.05)；其表达程度与肿瘤组织学分级及生存率密切相关(P<0.05)，与病人性别、年龄、肿瘤大小、部位及术后复发率等无明显相关。TIMP-1在MPNST中阳性表达率为41.4％， .卜庆队科大学倾l论文表达水平低于正常神经及其良性肿瘤中的表达，但无扯著性差异;其表达程度与病人性别、年龄、肿瘤大小、部位、组织学分级、术后复发率及生存率等均无明显相关。(3)在有随访资料的28例中，MMP一2表达分级超过TIMP一2表达分级者15例，MMP一2表达分级低于TIMP一2表达分级者7例，两组在术后复发率间无显著差异，在生存率间有显著差异(P<0.05);MMP一9表达分级超过TIMP一1表达分级者10例，MMP一9表达分级低于TIMP一l表达分级者8例，两组在术后复发率及生存率间均无撇著差异。 结论:MMP一2、TIMP一2的表达与MPNST的组织学分级及生存率有关。MMP一2表达卜调和rIMP一2表达卜调可能在MPNST的发生、发展过程中起重要作用。MMP一2、T 1 MP一2可作为判断MPNST恶性程度及预后的有用的参考指标。更多还原

【Abstract】 Objective: To investigate the relationship of the expression of gelatinases(MMP-2 and MMP-9) and their tissue inhibitors(TIMP-2 and TIMP-1) to clinicopathological characteristics and prognosis of MPNST.Methods: S-P immunohistochemical staining was used to detect the expression of MMP-2, MMP-9, TIMP-2, TIMP-1 in 58 MPNST, and the patients were followed-up.Resu I ts: (1) The expression of MMP-2 in MPNST was markedly higher than that in normal nerves and benign peripheral nerve sheath tumors(P<0.05). Increased MMP-2 reactivity was significant for histologic grade and survival time(P<0.05), but not for sex, age, tumor size, site or recurrence. The expression of MMP-9 in MPNST was higher than that in normal nerves and benign peripheral nerve sheath tumors, but no significant association was found between them. No significant association was found between the expression of MMP-9 and sex, age, tumor size, site ,histologic grade, recurrence , or survival time. (2) Theexpression of T1MP-2 in MPNST was markedly lowerer than that in normal nerves and benign peripheral nerve sheath tumors(P<0.05). Lack of TIMP-2 expression was significant for histologic grade and survival time(P<0.05), but not for sex, age, tumor size, site or recurrence. The expression of TIMP-1 in MPNST was lowerer than that in normal nerves and benign peripheral nerve sheath tumors, but no significant association was found between them. No significant association was found between the expression of TIMP-1 and sex, age, tumor size, site ,histologic grade, recurrence , or survival time. (3) Unbalance expression between MMP-2 and TIMP-2 was significant for survival time(P<0.05), but not for recurrence. Unbalance expression between MMP-9 and TIMP-1 was significant for neither recurrence nor survival time.Cone I us i ons: The expression of MMP-2 and TIMP-2 is associated with histologic grade and survival time of MPNST. Increased MMP-2 reactivity and lack of TIMP-2 expression may play a role in occurrence and progress of MPNST. MMP-2 and TIMP-2 might be useful to define aggressiveness and prognosis of MPNST.更多还原