脂质诱导的胰岛素抵抗对大鼠糖脂代谢的影响

【作者】 梁泽容；

【导师】 杨刚毅；

【作者基本信息】 重庆医科大学 ， 内科学， 2004， 硕士

【摘要】 目的 建立清醒状态大鼠扩展高胰岛素—正常血糖钳夹技术，并准确评价机体在体组织对胰岛素（Ins）的敏感性和胰岛素介导的糖代谢变化。方法 采用3-3H标记葡萄糖作为示踪剂建立自由状态下大鼠扩展高胰岛素—正常血糖钳夹技术，并动态观察其体内糖代谢的改变及血浆游离脂肪酸（FFA）和胰岛素（Ins）浓度随时间变化的过程。结果 （1）大鼠在4.8mU/kg.min的Ins速率输注下，血糖稳定在正常水平而肝糖输出被抑制，胰岛素介导的机体葡萄糖利用率较基础状态显著增加，血浆游离脂肪酸（FFA）浓度显著下降。（2）该钳夹术平均血糖、葡萄糖输注率（GIR）和葡萄糖利用率（GRd）变异系数分别为2.01%,4.5%和8.56%；重复试验GIR和GRd误差范围为1.4%和1.9%。结论 以3-3H标记葡萄糖作为示踪剂建立的自由状态下大鼠扩展高胰岛素—正常血糖钳夹技术具有准确、可靠的优点。在外源性胰岛素—葡萄糖代谢的稳定状态下，机体对葡萄糖利用显著增加，肝糖输出被完全抑制，脂肪分解显著减少。更多还原

【Abstract】 Objective To establish an extended hyperinsulinemic euglycemic clamp technique in conscious rats, and to evaluate the insulin sensitivity and insulin-mediated glucose metabolism in vivo.Methords An extended hyperinsulinemic euglycemic clamp technique with 3-3H labelled glucose as a tracer was applied in conscious rats to investigate the time course of plasma free fatty acids(FFA), insulin levels and glucose turnover during the insulin clamp.Results When insulin was infused at a rate of 4.8mU/kg.min，hepatic glucose production(HGP) was completely inhibited, and the concentration of FFA was remarkably decreased while insulin-mediated glucose disappearance rates (GRd) was significantly increased compared with basal values. The average coefficients of variation in blood glucose levels, glucose infusion rates(GIR)and glucose disappearance rate(GRd) were 2.01%.4.5% and 8.56% respectively. The errors of replication in GIR and GRd were 1.4% and 1.9% respectively.Conclusion Extended hyperinsulinemic euglycemic clamp technique with 3-3H labelled glucose as a tracer in the conscious rats were performed with good precision and credibility. During the steady-state of exogenous insulin and glucose metabolism, the insulin-mediated glucose <WP=8>disappearance rate(GRd) was significantly increased while the hepatic glucose production(HGP) was completely inhibited,and the lipolysis was remakablely decreased.更多还原