【作者】 贺岩；

【导师】 王守才；

【作者基本信息】 中国农业大学 ， 作物遗传育种， 2004， 硕士

【摘要】 玉米细菌性褐斑病是由于丁香假单胞菌丁香致病变种侵染而发病。目前，对于此病的遗传机制和分子水平的研究国内外还没有报道。本文以对玉米细菌性褐斑病高度免疫的自交系F₃₄₉和感病自交系P₂₅的杂交后代构建的重组自交系群体为材料，利用数量遗传分析方法研究了F₃₄₉的抗病遗传规律；选择极端抗感系构建抗感近等基因池，以亲本作对照，采用SSR标记技术，从池间筛选表现多态性的分子标记。获得连锁标记后，用重组自交系群体进行连锁分析和抗病基因的定位，并构建了抗病基因附近的玉米SSR标记连锁图谱。研究结果为进一步对该基因的克隆和功能研究奠定了基础。主要结果总结如下： 1．通过对玉米病叶病症、病原形态观察及鉴定分析，确定了在感病亲本P₂₅上侵染的病害类型是细菌性褐斑病，病原是丁香假单胞菌丁香致病变种。 2．制定了对玉米细菌性褐斑病田间调查方法。对田间性状调查结果进行抗性分析，经适合性检验符合孟德尔单基因控制的遗传分离规律。在F₉家系中的表现与F₈家系一致，结合F₁代田间表现，初步证实自交系F₃₄₉的抗病性是受一对显性基因控制的质量性状。 3．从覆盖染色体组的640对SSR引物中筛选出2对在抗感池中表现出差异的引物，分别为umc2409和bnlg1318，并都位于第四染色体4.01-4.02附近。由于该基因是首次定位，暂定名为Psy01-F₃₄₉。 4．玉米抗细菌性褐斑病基因被定位于第4染色体短臂上，位于SSR标记umc2409和bnlg1318之间，与它们的遗传距离分别为7.6cM和12.2cM。更多还原

【Abstract】 Maize bacterial brown spot disease is caused by the infection of Pseudomonas syringae pv.syringae Van Holl. There is no report on cell and molecular genetics of this disease up to now. A Fg recombinant inbred lines (RILs) population which was constructed by the descendent of F349 (immune inbred line) and P25 (susceptible inbred line),as materials accompanied by the method of quantitative heredity, was used to study the heredity of resistance to this disease. The resistant-susceptible pools was constructed by choose the extremely resistant and susceptible plants and the technique of SSR was applied to select the molecular markers which revealed the difference between the parents. After obtaining the linkage maker, the study of linkage analysis and gene location using RILs was made. Besides, a genetic map was constructed in the vicinity of the resistant gene. The results obtained from this study provide a basis for isolating the resistant gene and studying its function. The main results are as follows.1. According to the symptoms and morphology of pathogeny, determined that the disease infested on the susceptible inbred line P25 was bacterial brown spot disease, and the pathogeny was Pseudomonas syringae pv.syringae Van Holl.2. Worked out the methods to evaluate incidence. According to the resistant analysis of the field investigation and X examination, proved that the genetic mechanism was in accordant with Mendelian monogene segregation law. Appearances in F9 families was consistent with F8 families. Associated with F1 field behavior, proved that the resistant character is controlled by dominant monogene.3.2 out of 640 SSR markers which distribute on the whole genome showed polymorphic between resistant and susceptible pools.These are umc2409 and bnlg1318, respectively. Moreover, all two makers were located on 4.01-4.02 site of chromosome 4. The name of this gene temporarily nominated is Psy01-F349.4. One resistant gene to bacterial brown spot disease was mapped on chromosome 4, and thedistance to umc2409 and bnlg1318 is 7.6 and 12.2cM, respectively.更多还原