【作者】 黄金光；

【导师】 李怀方；

【作者基本信息】 中国农业大学 ， 植物病理学， 2004， 硕士

【摘要】 本实验对几种表现病毒病症状的木本植物样品(包括扶桑、白蜡、丁香、泡桐和枣树)采用生物学、血清学、电镜和分子生物学等技术进行了病毒检测与鉴定。综合各种检测方法得到如下结果：河南五彩扶桑为番茄花叶病毒(ToMV)侵染；北京扶桑、宁夏大叶白蜡、北京大叶白蜡与丁香为烟草花叶病毒(TMV)侵染；河南泡桐为TMV和黄瓜花叶病毒(CMV)复合侵染；枣树花叶病的病原没有得到明确的结论。 ToMV侵染扶桑、TMV侵染扶桑和丁香以及TMV与CMV复合侵染泡桐均为首次报道。 以林业微生物菌种信息为对象，采用计算机网络和数据库技术，构建了基于Web的林业微生物菌种数据库查询系统，主要研究内容包括：确定了架构本系统的IIS5.0+ACCESS+ASP的合理方案。建立了结构适宜的林业微生物菌种数据库和开发系统所需的软硬件结构。设计了高效的菌种信息数据库检索程序，实现了包括菌种菌号、拉丁学名等10个相关信息的模糊查询功能。用Dreamweaver UltraDev 4.0网页开发管理工具设计了系统的网站，并集成了菌种资源查询系统。 基于Web的查询系统能为普通用户提供翔实的菌种信息，并且实现了菌种信息的动态管理，提高了工作效率和工作水平，具有较高的的实用价值。更多还原

【Abstract】 Some forestial plants infected by viruses, including Hibiscus rosa-sinensis Linn., Fraxinus chinensis, Syringa oblate Lindl., Paulownia and Zizyphus jujube Mill., were detected and identified by bioassay, serology and molecular biological techniques. According to the results of these detection, it revealed that, one Hibiscus rosa-sinensis sample from Henan was infected by ToMV, the other sample from Beijing was infected by TMV, two Fraxinus chinensis samples respectively coming from Ningxia and Beijing were infected by TMV, Syringa oblate Lindl.sample from Beijing was also infected by TMV, Paulownia sample from Henan was infected by CMV and TMV. We are still under the detection and identification about the pathogen from Zizyphus jujube Mill. now.It were firstly reported about ToMV on Hibiscus rosa-sinensis Linn., TMV on Hibiscus rosa-sinensis Linn, and Syringa oblate Lindl., CMV and TMV on Paulownia.Taking the forestry microorganisms information as application object, based on computer network and database technology, this dissertation present a research on designing of database index searching for forestry microorganisms. The research consists of such items: The rational scheme of IIS5.0+ ACCESS+ASP was applied to the system. The database of forestry microorganisms was built correctly, and the frameworks of the system’s hardware and software were made. The effective retrieve program for the database was designed. A website that integrated with database index searching for forestry microorganisms system was designed, using Dreamweaver UltraDev 4.0, a website development tool.The system can provide full and accurate forestry microorganism index for the users and provide functions of dynamically managing the database for the administrator.更多还原