【作者】 王毓；

【导师】 张军；

【作者基本信息】 中国农业大学 ， 植物学， 2004， 硕士

【摘要】 本文以中花8号水稻细胞悬浮体系为材料，研究了抗逆诱导剂IPT、ABA处理对不同激发子诱导水稻细胞PAL、LOX基因表达状况的影响。从而比较IPT、ABA在水稻抗逆诱导过程中作用机制的异同。 实验中首先建立了稳定的水稻悬浮细胞系。确定了中花8号水稻悬浮细胞培养的适宜2，4-D浓度为3.0mg／L，适宜pH为6.0。利用PAL酶活作为指标，确定本实验体系中真菌激发子的适宜浓度为10mg／L，IPT的最佳浓度为5μmol／L，并发现水稻悬浮细胞经过IPT的处理作用，提高了其PAL酶途径对于真菌激发子诱导的敏感性。 在不同激发子诱导下，比较5μmol／L IPT和5μmol／L ABA处理对水稻细胞PAL、LOX基因表达状况的影响。结果发现：在无激发子存在的条件下，IPT本身可以诱导水稻细胞的PAL和LOX两种基因的表达，而ABA只能诱导LOX基因的表达；在真菌激发子存在的条件下，IPT处理细胞能够增强PAL基因的表达，却不再诱导LOX基因的表达，ABA处理细胞的PAL和LOX两种基因都不能被诱导表达；在盐激发子存在的条件下，IPT、ABA两种处理都能够增强水稻细胞的PAL和LOX基因的表达。 以上结果表明，IPT能够增强真菌激发子和盐激发子诱导下水稻细胞的PAL和LOX基因表达，而ABA只能在盐激发子诱导下增强水稻细胞的PAL和LOX基因表达。更多还原

【Abstract】 The expression of PAL and LOX genes in the suspension cells of rice that treated with IPT and ABA to the elicitors of fungal and salt was studied.The steady rice suspension cells system was established.The best concentration of 2,4-D was determined to be 3.0 mg/L,and the best pH was 6.0. The appropriate concentration of the fungal elicitor in this system was 10mg/L,and the best concentration of IPT was 5 u mol/L. IPT enhanced the suspension cells’ susceptivity to the low dose of elicitor.After the different elicitors were added, the effects on the expression of PAL and LOX genes in the suspension cells treated with 5 u mol/L IPT and 5 u mol/L ABA were compared . As a result,when there was no elicitor,the treatment singly with IPT can induce the expression both of the PAL and LOX genes,while the treatment with ABA can simply induce the expression of the LOX gene. When the fungal elicitor was added, the cells treated with IPT can enhanced the expression of the PAL gene,but the expression of the LOX gene in the cells was disappeared; the treatment with ABA can induce the expression of neither the PAL gene nor the LOX gene. And when the salt elicitor was added, the expression of PAL and LOX genes were enhanced observably in the cells treated with IPT and ABA.All the results above indicated that IPT can enhanced the susceptivity of PAL and LOX of the suspension cells of rice, while ABA can induce just the way of LOX .更多还原