【作者】 李传业；

【导师】 滕光辉；

【作者基本信息】 中国农业大学 ， 农业生物环境与能源工程， 2004， 硕士

【摘要】 应用先进的环境调控技术，实现组培苗的自动化、规模化、工厂化生产是使组培苗成为商品普遍应用于农业的较好途径。然而，目前培养环境内各环境因子对培养植物的生长发育及其形态的影响，还有许多问题没有弄清，相关环境控制系统急待开发。本研究的目的是开发一套能长期自动直接监控组培微环境CO₂浓度的调控系统；初步探索对组培箱内气体净化、除湿的方法；探索CO₂富集对组培苗生长发育的影响，为日后的科学实验和生产实践提供借鉴。 本研究开发了一套基于PLC的无糖组培微环境CO₂增施监控系统。研究内容包括：高压高纯度CO₂施放模糊控制器研究，实现对组培环境的CO₂浓度以及相对湿度的监控；进行生根阶段无糖培养的CO₂富集(富集浓度900～1100μmol·mol^-1)与无CO₂富集的对比实验，研究分析CO₂富集对组培苗的影响；初步尝试用箱外循环法对组培箱内的空气进行除湿净化处理。 结果表明：所研制的无糖组培微环境CO₂增施监控系统工作正常、稳定、可靠，能有效地将组培箱内的CO₂浓度及其相对湿度控制在设定范围内；用箱外循环法对箱内气体净化处理后，改善了组培箱内的气体环境，有利于组培苗的生长发育；CO₂富集环境中，组培苗生长健壮、发育良好，生根率显著提高，根系发达。本实验条件下，处理组菊花组培苗的新增叶片数是对照组的1.85倍；处理组的根总长是对照组的10.6倍；处理组根数是对照组的6.67倍。更多还原

【Abstract】 It is a good way for plantlets’ commercial application in agriculture to develop automated industrial-scale culture facilities by using advanced environmental control technology. But there are a lot of complexed problems about the environmenteal factors effecting the growth and morphasis of plantlets, so it is urgent to develop environmental control system which adapt to use in plant tissue culture . The object of this research is to develop an environmental control system which can directly monitor and control the micro-environment in which plantlets were planted during the whole period of the culture;seek the way to putrify the air in culture box;study the effects of CO2 enrichment on culture plantletoWe designed an environmental control system for sugar-free plant tissue based on PLC. In this paper introduced the study of fuzzy controller which was used for CO2 supplying control from CO2source-high-pressure steel tin to culture box, studied the control of relative humidity and CO2concentration in the culture box and effects of CO2 enrichment (enrichment concentration was 900-1100mol mol-1) on the growth of chrysanthemum plantlet and tried to clean the air in the culture box by cyclingThe results indicated that this environment control system operates normally and reliably .It can keep the CO2 concentration and relative humidity in setting scope; it is good to the growth of the culture plantlets to clean the air in culture box by cycling; CO2 enrichment can promote the growth and photosynthetic and can increase the percentage of shooting The number of new leaves of the chrysanthemum plantlet cultured under CO2-enriched condition is 1.85 times of the plantlet cultured under CO2-nonenriched condition; The number of the roots of the chrysanthemum plantlet cultured under CO2-enriched condition 5.67 times greater compared with the plantlet cultured under CO2 nonenriched condition. The sum lengths of the roots of chrysanthemum plantlet cultured under CO2-enriched condition were 10.6 times as great as the plantlets cultured under CO2-nonenriched condition.更多还原