【作者】 李海云；

【导师】 张肇铭；

【作者基本信息】 山西大学 ， 环境科学， 2004， 硕士

【摘要】 针对我国现有多数污水处理设施只去除有机物和固体悬浮物，不具有脱氮功能，我们在试验中开发了一种简捷、高效的脱氮微生物制剂，将其直接投入处理设施的曝气池中，使得能够实现脱氮的目的。 脱氮微生物制剂的制备是将亚硝酸菌、反硝化菌的高密度培养液高速离心，菌体浓缩物进行适当配比，用PVA载体固定而成。试验研究了亚硝酸菌和反硝化菌的高密度培养条件、用PVA固定脱氮菌的方法、实现高效脱氮的环境条件、脱氮微生物制剂处理实际废水的效果。试验结果表明： 一、用硅胶平板分离亚硝酸细菌的富集培养液，分离得到亚硝化球菌和亚硝化单胞菌，对分离到的这两株菌扩大培养六周后，进一步组合筛选，得到氨氧化速率高的混合菌株，应用正交试验法对其高密度培养条件进行试验，得到高密度培养的最适条件：NaHCO₃为lg/L，（NH₄）₂SO₄为2g/L，pH值8.0，微量元素为1ml/L及溶解氧大于2.4mg/L。在此条件下，亚硝酸细菌的高密度培养液菌体浓度达到1.4×10⁹MPN/ml。 二、在亚硝化型反硝化菌体富集培养液中，筛选出三株脱氮率高的菌株。这三株菌体的等比混合物为脱氮率最高的种型，研究结果表明：采用乙酸钠为碳源和能源富集培养反硝化细菌比采用甲醇、乙醇或酒石酸钾钠更易分离筛选出高效生物脱氮菌种；试验菌种在脱氮过程中没有硝酸盐的生成；反硝化菌的最适高密度培养条件为接种量10％，C/N 2.5，pH值7.0～8.0之间，温度30℃。 三、采用聚乙烯醇（PVA）-硼酸包埋固定化法，选用PVA为包埋载体，粉末活性炭作为无机载体，包埋固定实验分离得到的亚硝酸菌和反硝化菌，制成固定化脱氮微生物颗粒。试验结果表明：当COD处于500～1000 mg/L内，pH值在8.0～8.5之间，温度和溶解氧分别是30℃和2～6mg/L，此时，NH₄-N去除率达90％以上。 四、以流化床作为生物反应器，采用SBR运行方式对实际城市生活污水进行处理试验。当颗粒填充率为10％，进水NH₄-N浓度为40mg/L，反应时间为8h时，氨氮去除率可达90％以上，TN的去除率为80％左右，同时COD的去除率约40％。更多还原

【Abstract】 Most traditional effluent disposal establishments only be able to removal organic pollutants and solid suspend but have not the function of nitrogen removal. As to the problems, a kind of convenient and efficient microbial preparation for nitrogen removal was developed in this paper. As it was directly cast into the aeration tank, the effluent disposal establishments is capable of the function of nitrogen removal.The microbial preparation for nitrogen removal was equipped by making the high density culture liquid of nitrous bacteria and denitrifying bacteria centrifugal, mixing condensate bacteria in appropriate proportion and immobilizing the mixture with polyvinyl alcohol (PVA). In this thesis, the condition of culturing the nitrous bacteria and denitrifying bacteria of high density was determined, the method of immobilization by PVA was established, the environment for realizing nitrogen removal at high speed was studied, and the result of using the microbial preparation for nitrogen removal to treat waste water was detectedOn the silicate plate, pure nitrobacteria were obtained. Strain Y1 was identified to be Nitrisospira sp. Strain Y2 was Nitrosococcua sp. By extending the two bacteria for 6 weeks and selecting further, strain mixed of high amide oxidation rate produced. The results showed that under the optimum condition: NaHCO3 1g/L, (NH4) 2SO4 2g/L, pH 8.5, DO>2.4mg/L, trace element solution 1 ml, the concentration of nitrobacteria was up to 1.4×109MPN /mlThree high denitrification bacteria were isolated from sludge enrichment culture. The mixture of the three strains in same share is the type of highest nitrogen removal rate. The results showed that the high affection removal nitrogen bacteria screened out by sodium acetate was more easily than using carbinol, ethanol, Kalium natriumtartaric acid as carbon and energy sources. And nitrate wasn’t produced in the course of nitrogen removal. The optimum condition for high-density cultures of denitrification bacteria was inoculation 10%, C/N 2.5, pH 7.0 to 8.0 and temperature 30℃.The immobilized microbes pellets were made from nitrosobacteria and denitrifying bacteria using polyvinyl alcohol(PVA)cross linked with boric acid ,PVA is embed carrier and powdered activated carbon is inorganic carrier. The source of nitrosobacteria and denitrifying bacteria come from activated sludge system. The results showed that while the COD between 500 and 1000mg/L,pH8.0 to 8.5 ,temperature 30℃ and DO 2 to 6mg/L,the removal efficiency -reached 90%.In biological fluidized bed reactor, Sequencing Batch Reactors system treating practical life wastewater was carried out. While the immobilized microbe’s pellets package percentage was 10%, influent NH4-N concentration was 40 mg/L, reaction time was 8h, the NH4-N removal efficiency reach 90%, the TN removal efficiency could reach 80%; the COD removal efficiency could reach 40% as well.更多还原