【作者】 张小红；

【导师】 王林和； 张国盛；

【作者基本信息】 内蒙古农业大学 ， 水土保持与荒漠化防治， 2004， 硕士

【摘要】 应用随机扩增多态性DNA（Random Amplified Polymorphic DNA, RAPD）标记法研究了内蒙古地区臭柏种群的遗传分化，经Popgen32 Version1.31统计分析，结果如下：用18条引物对臭柏4个种群进行扩增，共检测到124个位点，其中多态位点100个，多态率达80.7%。种内的Nei’s多样性指数和Shannon’s多样性指数分别为0.286和0.428，各种群内的平均多样性值分别为0.232和0.347，各种群的多样性变化趋势为毛乌素沙地（0.258，0.386）＞阴山山脉西段（0.258，0.376）＞浑善达克沙地（0.231，0.345）＞贺兰山（0.184，0.281）。臭柏种群总的基因多样性（Ht=0.288）大于种群间的基因多样性（Hs=0.233），种群间的遗传分化系数Gst=0.183，即种群间有一定的遗传变异，占总遗传变异的18.3%，种群内的遗传变异占总遗传变异的81.7%。4个种群之间有比较相似的遗传多样性，由于地理隔离，也存在着一定的种群间分化。臭柏种群间的平均遗传距离为0.0865，平均相似性系数为0.9181，根据遗传距离进行UPGMA 聚类，毛乌素沙地和阴山山脉种群在1.928处相聚；浑善达克沙地与它们在2.366处相聚；然后它们与贺兰山种群在6.426处相聚，当把阈值取到2.4时，就可以聚为两类了，这主要与它们的生境有关。更多还原

【Abstract】 Genetic differentiation of Sabina vulgaris populations in Inner Mongolia was studied with Random Amplified Polymorphic DNA (RAPD) markers. After analyzed by Popgen32 Version1.31, the results were as follows.Through the amplification with 18 random primers, 124 repeatable loci in which 100 were polymorphic were detected. Percentage of polymorphic loci was 80.65%. Nei’s index and Shannon’s index were 0.286 and 0.428 respectively, and they were 0.232 and 0.347 within populations respectively. Nei’s index and Shannon’s index in each population were Mu Us sandland（0.258,0.386）＞Yinshan mountain（0.255,0.376）＞Hunshandake sandland（0.231,0.345）＞Helan mountain（0.184,0.281）.The total gene diversity was 0.288. The gene diversity within populations was 0.233. The coefficient of genetic differentiation was 0.183. The genetic variation among populations was 18.3%, and within populations was 81.7%. The genetic diversities in 4 populations were similar, but the genetic differentiation also existed because of geographic isolation.The average genetic distance and Nei’s genetic identity respectively were 0.0865 and 0.9181 among populations. By cluster analysis (UPGMA) based on unbiased Nei’s genetic distance, the populations in Mu Us sandland and Yinshan mountain clustered at 1.928, then they clustered at 2.366 with population in Hunshandake sandland. At last, they clustered at 6.426 with Helan mountain. They were two clusters when the threshold was 2.4, which was correlative with habits.更多还原