【作者】 高娃；

【导师】 呼和巴特尔； 吴树清；

【作者基本信息】 内蒙古农业大学 ， 预防兽医学， 2004， 硕士

【摘要】 本研究通过对内蒙古乌兰察布地区绵羊感染的消化道线虫优势虫种—细颈线虫（Nematodirus spp）对无角多赛特羊和当地蒙古羊的感染率和感染强度进行对比研究，查明了这两个品种羊感染的不同状况。并对这两个品种羊的全血进行白细胞分离、流式细胞仪检测CD2、CD14的表达率，了解与细胞免疫有关的活性T细胞和与非特异性免疫有关的单核细胞的表达情况。对这两个品种羊的全血进行嗜酸性粒细胞计数（EO值），来考察两种羊抗病性的各自特点。1．无角多赛特羔羊的平均EPG值与当地蒙古羔羊的平均EPG值有极显著的差异（P<0.001）。无角多赛特育成羊所感染的细颈线虫虫卵的平均EPG值与当地蒙古育成羊的平均EPG值也有显著差异（P<0.05）。结果说明，无角多赛特羊的羔羊和育成羊对细颈线虫的抗感染性较弱。2．无角多赛特羊血液CD2表达率（23.32±7.50）低于对照组蒙古羊血液CD2表达率（39.97±6.02），t检验P<0.05，两组间差异有显著意义。在同一品种不同年龄段之间的比较中，羔羊组血液CD2表达率低于育成羊组，t检验P<0.05，两组之间的差异也有显著意义。无角多赛特羊血液CD14表达率（51.02±16.14）低于对照组蒙古羊血液CD14表达率（66.44±11.70），t检验P<0.05，两组间差异有显著意义。在同一品种不同年龄段之间的比较中，羔羊组血液CD14表达率低于育成羊组，t检验P<0.05，两组之间的差异也有显著意义。从而验证了无角多赛特羊和当地蒙古羊免疫水平上的差异。3．嗜酸性粒细胞计数（EO值）结果说明，嗜酸性粒细胞变化即EO值增多与细颈线虫感染强度多少相吻合，进一步证明了无角多赛特羊和蒙古羊抗感染情况的不同。更多还原

【Abstract】 In this study, surveyed and researched infectionsity and infection rate of Nematodirus spp, which is dominant socials in nemathelminthes of digestive tract in sheep on pasture of Wulanchabu of Inner Mongolia. Declared that the status of Nematodirus spp infection is different from Poll Dorset (experimental group) and Mongolian sheep (control group). Meanwhile, processed eosinophilicgranulocyte count test and measured the expression rate of CD2、CD14 cells by FCM.1. EPG value of lambs of experimental group was high than that of lambs of control group（P<0.001）.EPG value of young sheep of experimental group was high than that of young sheep of control group（P<0.05）.So the result showed that anti-infection for Nematodirus spp is weaker in Poll Dorset than in Mongolian sheep.2. The expression of CD2 of experimental group（23.32±7.50） was lower than that of control group（39.97±6.02）,and have significance（P<0.05）.At the same time, according to the age, the expression of CD2 of lambs was lower than that of young sheep,and have significance（P<0.05）.The expression of CD14 of experimental group （51.02±16.14）was lower than that of control group（66.44±11.70）,and have significance（P<0.05）. At the same time ,according to the age ,the expression of CD14 of lambs was lower than that of young sheep , and have significance（P<0.05）.The result testified the difference of anti-infection for Nematodirus spp between Poll Dorset and Mongolian sheep.3. Eosinophilic granulocyte count showed that Nematodirus spp infectionsity in digestive tract presents positive correlation with EO value.更多还原