葛根地连汤水溶性多糖的研究

【作者】 王晓娟；

【导师】 曾和平； 王辉；

【作者基本信息】 华南师范大学 ， 有机化学， 2004， 硕士

【摘要】 葛根地连汤复方中草药是由葛根、地黄、黄连、甘草四味中药组成，粉碎后用热水提取，乙醇沉淀，并经Sephadex G-150柱层析，得三种褐色精制多糖PRC-1，PRC-2；PRC-3(PRC为葛根地连汤水溶性多糖的简称)，研究表明均为均一组分多糖；同时经Sephadex G-200柱层析测得各多糖的平均分子量；运用HPLC法测定了PRC各多糖的单糖组成；并采用IR，高碘酸氧化和Smith降解推知其初级结构。 PRC-1、PRC-2、PRC-3各多糖的平均分子量(Mw)分别是12.2×10~4、2.5×10~4、7.62×10~3；PRC-1多糖分子中同时存在α和β糖苷键；PRC-2、PRC-3多糖分子中存在α糖苷键。单糖组成：PRC-1中含有L-鼠李糖(35％)、D-木糖(11.5％)、L-阿拉伯糖(11.5％)、D-葡萄糖(35％)、D-半乳糖(7％)；PRC-2中含有L-鼠李糖(5.3％)、D-木糖(2.8％)、D-果糖(16.3％)、D-葡萄糖(73.9％)、D-半乳糖(1.7％)；PRC-3中含有L-鼠李糖(12.4％)、D-木糖(16％)、L-阿拉伯糖(11.3％)、D-果糖(43％)、D-葡萄糖(17.3％)。PRC-1糖苷键连接方式主要以1→6和1→2方式连接；PRC-2糖苷键连接方式以1→2方式连接为主；PRC-3是1→6连接方式。更多还原

【Abstract】 Three kinds of polysaccharides were obtained from the Pueraria locata, Rehmannia glutinosa and Copis chinensis by water-extracting, alcohol-precipitating and Sephadex G-150 column chromatrography-separating. The results showed that they were homogeneous polysaccharides. Simotaneously, the molecular weight were obstained by Sephadex G-200 column chromatrography. The compositions of their single sugar were measured by HPLC. The basic structure was analyzed by HPLC, IR, periodate oxidation and Smith degeneration.Mw(PRC-l)=12.2 X 104, Mw(PRC-2)= 2.5 X 104, Mw(PRC-3)=7.62 X 103; PRC-1 consists of L-Rhamnose(35%), D-(+)-Xylose(l 1.5%), L-(+)-Arabinose(l 1.5%), D-Glucose(35%),D-Galactose(7%); PRC-2 concludes L-Rhamnose(5.3%), D-(+)-Xylose(2.8%), D-Fructose(16.3%), D-Galactose(1.7%), D-Glucose(73.9%); there are five kinds of single sugar including L-Rhamnose(12.4%), D-(+)-Xylose(16%), L-(+)-Arabinose(11.3%), D-Fructose(43%), D-Glucose(17.3%) in PRC-3. The data of IR spectrum revealed that PRC-1 is linked by α - glycosidic bonds and β -glycosidic bonds, PRC-2 and PRC-3 are linked by α - glycosidic bonds, while the backbone of PRC-1 is mainly linked by 1-6 and 1-2 glycosidic bonds, with a few 1-3 linkage, the backbone of PRC-2 is mainly linked by 1-2 glycosidic bonds, with a few 1-3 and 1-4 linkage, the backbone of PRC-3 is mainly linked by 1-6 glycosidic bonds, according to the results of periodate oxidation and Smith degradation.更多还原