人参川芎注射液对大鼠局灶性脑缺血疗效及机制的研究

【作者】 韩辉；

【导师】 涂晋文；

【作者基本信息】 湖北中医学院 ， 中医内科学， 2004， 硕士

【摘要】 目的： 观察人参川芎注射液对局灶性脑缺血模型大鼠神经的保护作用，并初步探讨其防治中风的作用机理。 方法： 通过电凝大脑中动脉建立脑缺血模型大鼠，设立假手术组、模型组、尼莫地平注射液组、川芎嗪注射液组、人参川芎注射液高、中、低剂量组，观察大鼠脑缺血后的神经功能、脑缺血梗死范围、缺血区形态学及超微结构的影响；以及血清中超氧化物岐化酶(SOD)的活性，丙二醛(MDA)含量，血浆中内皮素-1(ET-1)、脑组织肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、纤溶酶原激活物抑制剂(PAI-1)、组织型纤溶酶原激活物(t-PA)的水平、脑组织EAA含量。 结果： 1、人参川芎注射液高、中、低剂量组均能显著改善局灶大鼠脑缺血的神经功能障碍，效果优于尼莫地平及川芎嗪(P＜0.05)；降低脑梗死范围，与尼莫地平和川芎嗪无明显差异(P＞0.05)；增加造模14天后大鼠的体重，疗效优于尼莫地平(P＜0.05)，与川芎嗪无明显差异(P＞0.05)。 2、模型组血清SOD活性显著降低、MDA含量显著升高(P＜0.01)。人参川芎注射液能提高血清中SOD的活性，作用优于尼莫地平和川芎嗪(P＜0.05)。降低血清中MDA的含量，与尼莫地平和川芎嗪组无明显差异(P＞0.05)。 3、模型组血浆ET-1含量显著高于假手术组(P＜0.01)，人参川芎注射液可以显著降低血浆ET-1水平，低剂量组与尼莫地平和川芎嗪无明显差异(P＞0.05)，中剂量组优于尼莫地平(P＜0.01)，与川芎嗪无明显差异，而高剂量组则优于尼莫地平(P＜0.01)和川芎嗪(P＜0.05)。 4、模型组脑组织TNF-α及IL-1β含量显著高于假手术组(P＜0.01)。人参川芎注射液可以显著降低脑组织TNF-α及IL-1β含量。人参川芎注射液高、中剂量组降低TNF-α含量优于尼莫地平及低剂量组(P＜0.01)，但与川芎嗪无明显差异(P＞0.05)，降低IL-1β与尼莫地平、川芎嗪无明显差异(P＞0.05)。 5、模型组血浆t-PA含量及活性显著低于假手术组(P＜0.01)，PAI-1显著高于假手术组(P＜0.01)。人参川芎注射液可以提高血浆t-PA含量及活性，降低PAI-1含量。人参川芎注射液提高t-PA含量及活性优于尼莫地平(P＜0.01)，但与川芎嗪无明显差异(P＞0.05)。 6、模型组大鼠脑组织Glu含量显著高于假手术组(P＜0.01)。人参川芎注射液可以降低脑组织中Glu的含量，与尼莫地平及川芎嗪相比，无明显差异(P＞0.05)。 7、人参川芎注射液可以促使缺血大鼠病理形态学改变及超微结构的改变。溯，匕中医学陇2001级硕士研究生毕业击文结论: 人参川首注射液对对局灶性脑缺血损伤具有很好的保护作用，且其综合作用优于临床常用的尼莫地平注射液组及川芍嗓注射液组。实验结果表明，人参川首注射液可通过调节血管舒缩因子，减轻自由基损伤，平衡紊乱的纤溶系统，减轻炎症反应，抑制兴奋性氨基酸含量，降低兴奋性毒性而达到治疗脑缺血损伤的疗效。更多还原

【Abstract】 Objective: To study the protective effect and the dose-dependence of Renshen Chuanxiong injection in treating focal cerebral ischemia in rats; and to explore the mechanism of preventing and curing the cerebral ischemia with the theory of Yi Qi Huo Xie of Traditional Chinese medicine.Methods: Model rats of cerebral ischemia, which were prepared by electionic solidified the middle cerebral artery, were randomly divided into seven groups: the sham control, the model control, the Ligustrazine-treated control, Nimodipine treated control, and three experimental groups of varying injection dose of Renshen Chuanxiong. The neurologic function, the area of necrosis, changes of tissue morphology, and ultrastrcuture of ischemia area, and the level of ET-1, t-P, PAI-1 in plasma , TNF-α, IL-1β,EAA in brain tissue and SOD, MDA in serum were evaluated.Results: 1. In all three experimental groups, injection of Renshen Chuanxiong reduced the neurological deficits and brain-infracted areas and increased the weight of the rat with local brain ischemia. The results were better than Nimodipine and Ligustrazine-treated group.2. In Renshen Chuanxiong injection groups, SOD activity significantly increased and MDA level considerably decreased, while in the model control group, the SOD activity decreased and the MDA level increased, the result was better than Nimodipine and Ligustrazine-treated group.3.Injection of Renshen Chuanxiong significantly decreased ET-1 level in plasma and the result of highest-doze group was better than Nimodipine and Ligustrazine treated group, but there was no significantly difference between low-doze group and Nimodipine and Ligustrazine treated group. The plasma ET-1 level of the model control group was higher than that of the sham control group.4.Injection of Renshen Chuanxiong significantly decreased TNF-α and IL-1β levels in brain tissue and the result was better than Nimodipine-treated group,but there was no difference between injection of Renshen Chuanxiong andLigustrazine-treated group. The TNF-α and IL-1 β levels of the model controlgroup were higher than those of the sham control group.5.Injection of Renshen Chuanxiong increased t-PA concention and activity and decreased PAI-1 concention. The t-PA concention and activity in the model control group was significantly lower than that of the sham control group, while the PAI-1 concention in the model control group was significantly higher than that of the sham control group.6.Injection of Renshen Chuanxiong decreased Glu level in brain tissue, there was no significantly difference between it and other comparative groups. The Glu level in the model control group was lower than that of the sham control group.7.Injection of Renshen Chuanxiong could lead to improvent of morphology and ultra-structure of rats with brain ischemia.Conclusions: Renshen Chuanxiong injection may protect from cerebral ischemia injury in rats, its synthetical propective effect is better than ligustrazine injection and nimodipine injection which is often applied in clinic. Through adjusting the diastolic factor of blood vessel, reducing free radicals harm,EAA in brain tissue, blancing the disorder of plasmin system and lowering antiinflammation action, Renshen Chuanxiong injection attain the effect of treating cerebral ischemia injury.更多还原