电针“命门”、“绝骨”对去卵巢雌鼠骨质疏松症防治作用的实验研究

【作者】 石政达；

【导师】 闫庆军；

【作者基本信息】 湖北中医学院 ， 针灸推拿学， 2004， 硕士

【摘要】 目的： 骨质疏松症(Osteoporosis，简称OP)是指在骨代谢过程中，骨吸收大于骨形成，出现以低骨量和骨组织的显微结构的退行性变为其特征，临床表现为骨脆性和骨折易感性增加的一种代谢性疾病。本课题在前期工作的基础上，运用生物免疫技术，从电针对去卵巢大鼠骨矿含量(BMC)、骨密度(BMD)、胰岛素样生长因子(IGF)调节角度，初步探讨电针治疗骨质疏松症的作用机制，为临床治疗骨质疏松症提供实验依据。 方法： 选用雌性未孕SD大鼠50只为实验对象，5月龄，体重180-200g。将其中30只摘除双侧卵巢进行造模，10只行假手术，另10只不做处理作为正常对照组。造模的动物随机分为去卵巢对照组、雌激素组、电针组，每组10只。雌激素组和电针组均在术后90天开始治疗，共4个疗程。末次治疗结束后，麻醉下检测腰椎和股骨的骨矿含量(BMC)和骨密度(BMD)，摘眼球取血，离心取上清，采用酶联免疫法检测胰岛素样生长因子-Ⅰ(IGF-Ⅰ)。 结果： 1．去卵巢雌鼠腰椎及股骨的BMC和BMD均下降(P＜0.01)； 2．去卵巢雌鼠血清IGF-Ⅰ含量下降(P＜0.05)； 3．雌激素和电针治疗均可提高去卵巢雌鼠腰椎的BMC、BMD，与去卵巢对照组比较有显著差异(P＜0.05)； 4．雌激素和电针治疗均可以提高去卵巢大鼠血清IGF-Ⅰ含量，与去卵巢对照组比较有极显著差异(P＜0.01)： 结论： 电针去卵巢大鼠“命门”，“绝骨”二穴，可以明显提高IGF-Ⅰ含量，可以明显提高去卵巢大鼠腰椎的BMC和BMD，提示电针治疗可直接或间接作用于成骨细胞，增强骨重建，改善骨质疏松，为临床运用电针治疗骨质疏松症提供了实验依据。更多还原

【Abstract】 Objective:Osteoporosis ( OP ) is syndrome shown on the bone absorptive rate is higher than formative rate during the metabolism of their bones. As the result, the progression of calcium deficiency and the degeneration of bone tissue are the characters in this period. Clinical manifestations appear on these elder persons, which are osteosclerosis and increasing the possibility of fracture. The approach used here is based on the former fundamental research. By using the molecular immunology technique, BMC ( Bond Mineral Content ) BMD ( Bond Mineral Density ) and IGF-I ( Insulin-like Growth Factor I ) was tested to study the mechanism of the electroacupuncture ( EA ) prophylaxis and treatment the OP. By conducting experiment, effects of different treatments were compared to see whether the difference exists, and provide experimental evident on clinical manifestation prophylaxis and treatment.Methods:In this experiment, 50 unfertilized female rats were chosen as the experimental objects, whose ages were all five-month-old and weight from 180 to 200 grams. By doing oophorectomy operations on 30 rats, fake operations on 10 rats, and non-operation on the rest of them as a normal group, the rats were randomly classified into three groups, 10 rats in each group. They were ovariectomized group, estrogen group, and EA group. Both EA group and estrogen groups were conducted treatments 90 days after the operations. All treatments were four-course. After the last treatment, the rats were anesthetized to test BMC BMD and taken blood by excising the rats’ eyeballs. The blood was centrifugated to get about lml serum to test IGF-I (Insulin-like Growth Factor I ) by ELISA ( Enzyme-linked Immunoadsordent Assay) .Results:1. Both BMC and BMD of ovariectomized rats’ lumbar vertebra and thighbone decreased ( P<0.01 ), so did IGF-I ( P<0.05 );2. Comparing with ovariectomized group, EA and estrogen could increase BMC and BMD of the lumbar vertebra and thighbone ( P<0.05 );3. Comparing with ovariectomized group, both EA and estrogen could enhance the level of IGF-I ( P<0.01 );Conclusion:According to the above results, both BMC and BMD of the ovariectomized rat’s lumbar vertebra and thighbone decreased, at the same time, IGF-I decreased evidently. EA not only can help the ovariectomized rats to increase BMC and BMD of the lumbar vertebra and thighbone, but also to increase IGF-I contents to form bone cells and improve osteoporosis problem. Compared with estrogen, EA lowers the negative side-effect, provides the entire comprehensive functions of traditional Chinese medicine, and provides new theory on clinical treatment.更多还原