【作者】 安红伟；

【导师】 李建吾； 余纪柱；

【作者基本信息】 河南农业大学 ， 蔬菜学， 2004， 硕士

【摘要】 本实验以河南农业大学园艺系提供的6份黄瓜自交系为试验材料，研究了弱光下黄瓜苗期光合指标、夜间呼吸强度、生理指标、形态指标的配合力，并对其进行遗传分析。根据其耐弱光的性能对6份材料进行分类。采用完全双列杂交Griffing方法Ⅱ法配制21个组合。1.研究了弱光单一因子条件下黄瓜苗期光补偿点、净光合速率和夜间呼吸强度的配合力及遗传规律，将6个亲本分类并得到2个较好的杂交组合。结果表明，在25℃的条件下，黄瓜苗期光补偿点的广义遗传力和狭义遗传力分别为71.75％和21.42％，净光合速率的广义遗传力和狭义遗传力分别为73.62％和0；20℃条件下夜间呼吸强度的广义遗传力和狭义遗传力分别为49.77％和20.59％。2.研究了正常温度（25℃/15℃）弱光条件下黄瓜苗期几个生理指标的配合力和遗传分析，其中包括超氧化物歧化酶（SOD）、过氧化物酶（POD）、多酚氧化酶（PPO）三种酶活性，丙二醛(MDA)含量，叶绿素a与b的比值(Chla/Chlb)五个指标，得到3个表现较好的亲本和5个组合。结果表明，在正常温度弱光条件下这5个指标的狭义遗传力均很低，遗传以显性效应为主。3.在弱光条件下，对黄瓜苗期所测的10个形态性状进行了遗传分析。茎粗、子叶面积、叶面积、比叶面积、地上鲜重、全株干重、下胚轴长和比下胚轴长这8个性状的狭义遗传力均不高，遗传以非加性效应为主。只有下胚轴长和比下胚轴长这两个性状的遗传符合加性-显性模型。更多还原

【Abstract】 Six cucumber inbred lines provided by horticulturedepartment of He Nan Agricultural university were used as materials in the experiment. The combining ability and genetic analysis of some characters of cucumber seedlings, including photosynthetic indexes , night respiration, physiological indexes and morphologic indexes ,were conducted by using complete diallel crossing Griffing Ⅱ.The six inbred lines were classified according to their tolerance to low light. 1.This paper studies the combining ability and genetic law of cucumber seedlings photosynthetic light compensation point, photosynthesis and night respiration We classified the six cucumber inbred lines according to their tolerance to weak light and found tow kinds of hybrid better than others. The results showed that the broad heritabilities of cucumber seedlings photosynthetic light compensation point and photosynthesis under 25℃ is separately 71.25% and 73.62%,the narrow heritabilities of which is separately 21.42% and 0.The broad heritabilities and narrow heritabilities of cucumber seedlings night respiration under 20℃ is separately 49.77% and 20.59%. 2.The combining ability and genetic analysis of several physiological indexes, including the activity of superoxide dismutase (SOD), the activity of peroxidase (POD)and polyphenal oxidase (PPO), the content of MDA and the ratio of chlorophyll a to chlorophyll b , were conducted by using complete diallel crossing Griffing Ⅱ . We found that three inbred lines and five kinds of hybrid better than others. The results showed that the narrow heritabilities of all the five indexes were very low under normal temperature (25℃/15℃) and weak light. 3. The genetic analysis were conducted for ten morphologic characters measured in cucumber seedlings under low light. The narrow heritabilities for eight morphologic characters were low, including stem thickness , cotyledon area ,true leaf area ,specific leaf area,fresh mass above ground , whole biomass , which were mainly dominant effect. The genetic model of stem length and specific stem length agreed with additive-dominant model.更多还原