【作者】 吴元锋；

【导师】 郑裕国；

【作者基本信息】 浙江工业大学 ， 生物化工， 2004， 硕士

【摘要】 井冈霉素是我国最大的一类农用抗生素，它对水稻纹枯病有很好的防治效果。我们对层析法分离井冈霉素各组分进行了研究，所用的树脂分别是阳离子交换树脂和阴离子交换树脂。 首先我们考察了从井冈霉素发酵液中提取井冈霉素的工艺，主要比较了几种树脂，其中以凝胶型的001×4树脂效果最好。我们进行了强酸性001×4树脂层析法分离井冈霉素A和B的研究，在最优条件下，井冈霉素A和B可以被分离出来。同时还进行了阴离子树脂层析法分离井冈霉素各组分的研究，所用的树脂是201×4。我们优化了工艺条件，并考察了洗脱流速、上样量、柱床高度和洗脱液pH对井冈霉素A、B保留行为的影响，优化后的10×400mm层析柱上的工艺为：上样量0.7克，柱床高度33.0cm，线速度19.1cm／h，以去离子水作为洗脱剂。在该工艺条件下，井冈霉素A、B、D和E可以被分离出来。然后对该工艺进行了放大，放大后收率为53.4％。对该分离方法建立了动力学模型，求出了两个参数K_bds[R+]和K_bds[ROH]，这两个参数均比较大，说明吸附力是比较强的。最后对井冈霉素B的酶解工艺进行了初步的研究。更多还原

【Abstract】 Jinggangmycins, which is effective fungicide against the sheath blight of rice plants, is the most widely used homemade farm-antibiotics. Experiments about separation of validamycin A and validamycin B with anion-exchange resin and cation-exchange resin were carried out.Cation-exchange resin 001X4 was used in our experiment. Optimum separation conditions were obtained when separation was carried out in 20 X 400 mm column: flow rate was 0.5mL/min, adder quantity was 0.7g and eluent was 0.1mol/L pyridine-acetic acid at pH value of 6.0.As to anion-exchange resin A600, the influences of elute flow rate, adder quantities, bed height, eluate pH value on separation of validamycin A and validamycin B were investigated. It was found that the influence of bed height on retention volume was the strongest, compared with other factors. The retention volume could decrease with increase of elute flow rate and adder quantity or decrease of bed height. Validamycin A and validamycin B could be separated effectively in the 10X400 mm column under the optimized conditions: elution flow rate of 19.1 cm/h, adder quantity of 0.7 g, bed height of 33.0 cm and used de-ion water as eluent. Except for Validamycin A and B, other minor components, Validamycin D and E were also separated from crude validamycin powder. The separation was also scaled up. Then a kinetic model was established to describe this procedure, two adsorption coefficients, KbdS[R+] and Kbds[ROH], were calculated. The value of these two coefficients was very high, so the main mechanism that contributed to separation was adsorption. At last, hydrolyzation validamycin B by enzymes were also carried out.更多还原