【作者】 冯素云；

【导师】 汪慧敏；

【作者基本信息】 浙江中医学院 ， 针灸推拿学， 2003， 硕士

【摘要】 目的 建立SD大鼠子宫内膜异位症模型，探讨隔药饼灸治疗子宫内膜异位症的可能作用机理。 方法 SD大鼠随机分为正常组和造模组，将造模组大鼠自体子宫内膜移植，建立子宫内膜异位症模型。造模结束后，随机分为模型组、隔药饼灸组、温和灸组、达那唑组，进行对比治疗。实验结束，用放免法测定各组大鼠血清IL-6和血浆PGE₂水平，并对异位子宫内膜病灶进行外观观察及病理组织学检查。 结果 ①模型组大鼠血清IL-6和血浆PGE₂水平比正常组明显升高，差异均有非常显著性意义（P＜0.01，P＜0.005）；②隔药饼灸、达那唑和温和灸三个治疗组与模型组相比，血清IL-6水平分别有不同程度的降低，差异均有显著性意义（P＜0.01，P＜0.01和P＜0.05），三个治疗组之间相比，差异无显著性意义（P＞0.05）；③三个治疗组的血浆PGE₂水平与模型组比较，分别有不同程度的降低，差异均有非常显著性意义（P＜0.005，P＜0.005和P＜0.01）；隔药饼灸与达那唑组间差异无显著性意义（P＞0.05）；但此二组与温和灸组相比，差异均有显著性意义（P＜0.05，P＜0.05）。④外观观察：三个治疗组异位内膜病灶体积，与模型组相比均有不同程度的缩小（P＜0.005，P＜0.01和P＜0.05），隔药饼灸与温和灸组间差异有显著性意义（尸<0.05）;囊肿结节萎缩或消失，表面血管明显减少等外观恢复率，隔药饼灸和达那哇两组与模型组比较，差异均有显著性意义（尸<0.01和p<0.05）;提示三种疗法均可明显改善异位病灶，以隔药饼灸和达那哇最为显著。⑤病理组织学检查显示:三个治疗组的子宫内膜腺体数目减少，腺体萎缩，间质细胞小而稀疏，有陈}日性出血点。 结论隔药饼灸可能通过降低血清IL一6和血浆PGE:水平，调节机体的免疫功能，抑制异位内膜的增生生长，促其萎缩等机制治疗子宫内膜异位症，其疗效与达那哇相仿，优于温和灸。此法不失为临床治疗子宫内膜异位症的有效途径之一。更多还原

【Abstract】 Objective To establish the experimental endometriosis in rats, to study the ectopic endometrum’s histological structure change and to explore the mechanism of the effect of moxibustion separated by herbal cake on the treatment of endometriosis.Methods 60 SD rats were randomly divided into two groups: the normal and the experimental. 51 SD rats were induced by surgery for the experimental endometriosis. After the model be made, rats in the experimental group were divided into endomrtriosis model group(EMM), moxibustion separated by herbal cake group(MSBHC), mild moxibustion group (MM), and Danazol group to treat for thirty days respectively. Then, the levels of Interleukin-6(IL-6) in the blood serum and prostaglandinE2 (PGE2) in the blood plasma of all groups were measured by radioimmunoassay(RIA); the ectopic endometrium’s focuses were observed by microscope and eyes.Results (1)Compared with the normal group, the levels of IL-6 in the blood serum and PGE2 in the blood plasma of EMM group were significantly higher (P<0.01 and P<0.005 respectively). (2)After treatment, the levels of IL-6 in the blood serum in the three treated groups were degraded significantly as compared with those of EMM group (P<0.01, P<0.01 and P<0.05 respectively), but without statistical meaning for the changes of IL-6 levels between these groups (P>0.05); (3)The levels of PGE2 in the blood plasma of three treated groups became obviously lower than those of EMM group, which showed very significance (P<0.005, P<0.005 and P<0.01 respectively); there was no difference between MSBHC group and Danazol group (P>0.05), but these two groups had differencefrom MM group(P<0.05, P<0.05 respectively ). (4)As eyes observing, the volume of ectopic endometrium in rats of three treated groups shrinked comparing with those of the EMM geoup; and there were significance between the MSBHC group and the MM group( P<0.01). The ectopic endometrium’s cysts or nodes shrinked or dissolved, the surface blood vessels reduced, so these showed that the three methods all could significantly improve the ectopic endometrium’s focuses (P<0.05) in observation for appearance, especially markedly in the MSBHC group and the Danazol group(.P<0.01, P<0.05 respectively ), but no difference between the two groups. (5)There were less counts shrinked glands, small glandular cavity and stromal cells and obsoleted blood dot by microscope observation in three treated groups.Conclusion It is suggested that the therapy of moxibustion separated by herbal cake similar Danazol might regulate the immune disturbance in endometriosis and inhibit the ectopic endomrtrium’s hyperplastic and develop, promoted their shrink atrophy by decreasing the levels of IL-6 in the blood serum and PGE2 in the blood plasma to treat endometriosis; so it’s one of effective methods in clinical practice for treantment on endometriosis and superior to the mild moxibustion method.更多还原