【作者】 邹立津；

【导师】 曾元临；

【作者基本信息】 江西医学院 ， 外科学， 2003， 硕士

【摘要】 目的 观察辐照氟银异体肌腱移植的效果，同时建立一种新的异体肌腱处理技术。方法 32只家兔随机分为4组：分别将制备好的辐照氟银异体肌腱（A组），单纯辐照异体肌腱（B组），深低温冷冻异体肌腱（C组）与自体肌腱（D组），行肌腱移植。术前进行各组微生物学检测；术前、术后对比观察生物力学及形态学组织学的变化；术后行各组免疫源性、羟脯氨酸含量测定。结果 ⑴与A组相比，单纯辐照组及深低温冷冻组抑菌作用极为微弱（P<0.01）。A组含银量高，而其余各组未检出。⑵术前辐照组最大载荷和抗张强度明显大于其它各组（P<0.01），但术后12周差别无统计学意义（P>.05）。⑶组织学变化：术后4周各处理组细胞增多，均多于自体组，A、B组细胞呈椭圆形，C组为线形或梭形；术后12周各处理组细胞减少，仍多于自体组，细胞以线形或梭形为主。⑷辐照组淋巴细胞死亡率与自体组无差异（P>0.05），均低于冷冻组（P<0.05）；术后12周辐照组羟脯氨酸含量与自体组无差异（P>.05），但高于冷冻组（P<0.05）。结论 辐照氟银异体肌腱具有明显的低抗原性、无菌且有强抑菌作用，可作为一种良好的自体肌腱替代材料。更多还原

【Abstract】 Objective To observe the effect of homograft of γ-Irradiated tendon incorporated with norfloxacin and silver immersion and develop a new homograft tendon processing technique. Methods 32 rabbits were randomly divided into the following four groups: irradiated allogeneic tendon incorporated with norfloxacin and silver group(Group A), irradiated allogeneic tendon group(Group B),deep-frozen allogeneic tendon group(Group C)and autogeneic tendon group(Group D).They were then implanted into defects of Achilles tendons respectively. Ag+ level and bacteriostatic effect were detected in vitro. Biomechanic or histologic properties were observed preoperatively and postoperatilely. Immunologic property and hydroxyproline content were determined postoperatively. Results (1)No bacterial growth was investigated in Group A and B.By contrast,tendons of A group can effectively inhibit growth of bacteria(P<0.01).(2) Before implantation, the ultimate tensile stress of Group A and B was markedly greater than that of Group C and D（P<0.01）,but there was no significant difference 12 weeks after operation.(3)Histological findings:At four weeks after transplantation,there were more cells in all grafts than in normal control tendons.The cells of the irradiated grafts were mainly oblong while others were predominatly spindle-shaped or linear. At 12 weeks,cellularity of all grafts had decreased but remained greater than in normal control tendons.The cells were mainly spindle-shaped or linear .(4) The contents of lymphocytoxicity showed lower in Group A,B and D than in Group C（P<0.05）.The Hyp level was no significant between Group A ,Band D（P>0.05）, but it was lower in Group C（P<0.05）.Conclusion γ-Irradiated tendon incorporated with norfloxacin and silver immersion is a good substitute for autogenous grafts,which can remarkably decreased the antigenecity with sterility and strong bacteriostatic effect.更多还原