【作者】 陈颖；

【导师】 陈国南；

【作者基本信息】 福州大学 ， 分析化学， 2004， 硕士

【摘要】 本论文着重研究了三种β2－受体激动剂：克伦特罗、西马特罗和沙丁胺醇的毛细管电泳?安培检测及电化学分析方法，以及中成药藿香正气水中主要有效成分厚朴酚与和厚朴酚的毛细管电泳?安培检测方法。在论文的第一章中，我们对β2－受体激动剂残留分析进展以及中成药有效成分含量测定方法作了概述。提出本论文所要从事的研究工作的设想。在论文的第二章中，我们采用了循环伏安法(CV)，线性扫描伏安法(LSV)，计时库仑以及恒电位库仑电解法等现代电化学技术详细研究了克伦特罗在玻碳电极上的电化学还原行为。在此基础上建立了灵敏的测定克伦特罗的方波溶出伏安法，并成功地应用于模拟兔血清及尿样中克伦特罗的测定，结果令人满意。在论文的第三章和第四章中，我们研究了西马特罗和沙丁胺醇在Nafion修饰玻碳电极上的电化学行为，建立了选择性好，灵敏度高的Nafion修饰电极微分脉冲溶出伏安法测定西马特罗以及方波溶出伏安法测定沙丁胺醇的分析方法，使得二者的检测限接近nM级，同时将该体系应用于模拟血清样及尿样中西马特罗和沙丁胺醇的检测，结果令人满意。并结合计时库仑，库仑电解等方法对西马特罗和沙丁胺醇在Nafion修饰玻碳电极上的电化学氧化反应机理进行了探讨，测定了两物质在Nafion膜上的扩散系数、选择性系数和分配系数。毛细管电泳－电化学检测技术对具有电化学活性的物质的分离检测具有极大的优越性。论文的第五章采用高效毛细管电泳－安培检测法对克伦特罗、西马特罗和沙丁胺醇进行了同时分离检测，上述三种化合物在16min内完全分离。该法重现性好，灵敏度高，线性范围宽，用于模拟饲料及尿样中克伦特罗、西马特罗和沙丁胺醇的分离测定，结果令人满意。在论文的第六章中，我们在研究厚朴酚及和厚朴酚基本电化学性质的基础上，采用毛细管电泳－安培检测法对霍香正气水中的厚朴酚及和厚朴酚进行了同时分离测定，两待测物在6min以内完全分离，对实际样品的测定获得良好结果。更多还原

【Abstract】 In this thesis, we have mainly studied on three kinds of β2－agonists, such as clenbuterol, cimaterol and sabutamol, for their simultaneous determination by capillary electrophoresis with amperometric detection(CZE-AD) and their electrochemical properties. We also develop a method for simultaneous determination of honokiol and magnolol in a traditional Chinese medicine, Huo Xiang Zheng Qi Shui, by CZE-AD. In section 1, the progress of the analytical methods for β2－agonists residues and Chinese traditional medicine was reviewed.In section 2, the electrochemical behavior of clenbuterol was investigated by cyclic voltammetry, linear sweep voltammetry chronocoulometry and bulk electrolysis with coulometry on a glassy carbon electrode. A simple, quick and sensitive method for detection of clenbuterol by Osteryoung Square Wave Stripping Voltammetry was developed. The method was applied to the assay of clenbuterol in rabbit serum and urine with satisfactory results.In section 3 and section 4, the electrochemical behavior of cimaterol and sabutamol on a Nafion modified electrode was investigated. A high selective and sensitive differential pulse voltammetry method for determination of cimaterol and a simple, quick and sensitive method for detection of sabutamol by Osteryoung Square Wave Stripping Voltammetry were developed, with a low detection limit in the sub nM range. This method was applied to the assay of cimaterol and sabutamol in rabbit serum and urine with good results. The ion-exchange selectivity coefficient, the apparent diffusion coefficient and the distribution coefficient were determined based on the data from experiments of chronocoulometry, bulk electrolysis with coulometry etc. <WP=4>Since capillary electrophoresis with amperometric detection has great superiority for separation and determination of electrochemical active substances, this method was applied to separation and determination of clenbuterol, cimaterol and sabutamol in section 5. Under the optimum conditions, above three analytes were separated completely within 16 min. The method has been employed for the determination of clenbuterol, cimaterol and sabutamol in rabbit urine and feed by standard addition with satisfactory results.In section 6, based on the investigation of the electrochemical characteristics of honokiol and magnolol, a method for simultaneous separation and detection of honokiol and magnolol in the traditional Chinese medicine, Huo Xiang Zheng Qi Shui, by capillary electrophoresis with amperometric detection was developed. Under the optimum conditions, the analytes were separated completely within 6 min, and the good results for the real samples were obtained.更多还原