【作者】 成永强；

【导师】 鲍所言；

【作者基本信息】 河北大学 ， 分析化学， 2003， 硕士

【摘要】 催化荧光动力学分析法结合了动力学分析法和荧光分析法的特点，具有高灵敏度和更高的专属性，是用于检测痕量组分的有力手段。本文主要研究了催化荧光动力学分析法在测定痕量无机离子钒（Ⅴ）、硒（Ⅳ）和有机物对苯二酚方面的应用；对荧光猝灭法测定痕量硒（Ⅳ）进行了研究。全文分如下部分： 1．对催化荧光动力学分析法的发展和应用进行了综合评述，并对其应用前景加以展望。 2．建立了催化荧光动力学分析法测定痕量无机离子(V⁵⁺和Se⁴⁺)的新方法。选择了最佳试验条件（温度、时间和试剂用量等），并对催化反应机理进行了初步探讨。 3．利用有机物（对苯二酚）对钒（Ⅴ）催化溴酸钾氧化吖啶橙反应体系的活化作用，测定了实际样品中对苯二酚的含量，研究了最佳试验条件，方法迅速、简便、选择性好。初步探讨了活化作用机理。 4．利用Se⁴⁺氧化碘化钾的反应产物使吖啶橙荧光猝灭，建立了荧光猝灭法测定痕量硒的新方法。方法简便灵敏，用于水、蒜样中硒（Ⅳ）的测定，结果满意。并探讨了反应机理。更多还原

【Abstract】 The kinetic analysis of catalytic fluorimetic is a effective method in solving trace amounts analysis with high sensitivity and higher selectivity because it synthesizes the characteristics of kinetic and fluorescence analysis. This paper focuses on the application of catalytic fluorimetry in determination of trace amount inorganic ion vanadium and organic compound. The determination of selenium(IV) by fluorescence quenching reaction is also studied.The paper consists of four parts. In part 1, the progress and application of catalytic fluorimetic analysis are reviewed. Application of the analysis and future study are prospected.In part 2, the new method is described for the determination of trace vanadium(V) and selenium(IV). The optimized conditions(temperature, time and the quantity of reagents etal.) are selected and the mechanism of the catalytic reaction has also been discussed.In part 3, a new method is developed for the determination of the concentration of activist in real samples. It is based on the activation effect of hydroquinone on the catalytic oxidation reaction of acridine orange by potassium bromate in the presence of vanadium( V ).The optimized conditions are studied. The method is quick, convenient and has high selectivity. The mechanism of the active reaction has been discussed.In part 4, a new fluorescence quenching method has been proposed for the determination of trace selenium. The method is based on the fluorescence quenching of acridine orange by the oxidation reaction product of selenium(IV) with potassium iodide. The method is simple and sensitive. It has been applied to the determination of water and garlic samples with satisfactory results. The mechanism of the reaction has also been discussed.更多还原