【作者】 高英；

【导师】 孙汉文； 杨莉丽；

【作者基本信息】 河北大学 ， 分析化学， 2002， 硕士

【摘要】 氢化物发生-原子荧光光谱法具有检出限低、灵敏度高和可同时多元素分析等特点在痕量和微量元素分析的工作中发挥着越来越重要的作用。本文主要研究利用氢化物发生-原子荧光光谱技术在测定中草药中的微量元素砷、硒、汞的应用。 全文分为： 第一章：描述了原子荧光光谱法基本原理，对九十年代以来原子荧光光谱法分析技术的发展及应用进行了综合评述。并对其应用前景加以展望，引用文献79篇。 第二章：利用氢化物发生-原子荧光光谱法测定中草药中微量砷的方法，研究了最佳条件，以5％硫脲+5％抗坏血酸为预还原抗干扰剂，成功地测定了十种中草药样品中的砷。 第三章：建立了氢化物发生-原子荧光光谱法测定中草药中的微量硒的分析方法。研究了仪器的工作条件、试剂对硒原子荧光强度的影响及硒(Ⅵ)的还原条件，探讨了共存离子对硒测定的干扰和消除方法，方法的检出限为0.21μg／L。利用本方法成功的测定了十种中草药中的微量硒，回收率为95～105％。方法简便、快速、灵敏、准确。 第四章：利用氢化物发生—双道原子荧光光谱法同时测定中草药中的微量汞和砷建立了氢化物发生-双道原子荧光同时测定中草药中汞和砷的新方法，选择了测定的最佳条件，研究了酸和还原剂的用量对测定中草药中As、Hg的影响以及共存元素的干扰情况。采用加标回收试验方法，验证了该法的准确性。实验结果表明，采用该法可快速、准确地测定中草药中微量有害元素As和Hg，结果令人满意。更多还原

【Abstract】 A method, hydride generation atomic fluorescence spectrometry, for the determination of trace elements places more and more role in many fields, for its low detection limit and high sensitivity. This paper focuses on the application of hydride generation atomic fluorescence spectrometry in determination of arsenic, mercury and selenium. The paper consists of four chapters.In chapter 1, the fundamental principle was described. The progress and the application of atomic fluorescence spectrometry are reviewed and the application of the method is prospected. Seventy-nine references are cited.In chapter 2, a method has been proposed for the determination of trace arsenic by hydride generation-atomic fluorescence spectrometry (HG-AFS). Effects of experimental conditions on the fluorescence intensity of Arsenic were investigated and optimized. The proposed method was successfully applied to the determination of arsenic in ten kinds of Chinese herbs. The advantages of the method were simple, fast, sensitive and accurate.In chapter 3, a method for the determination of selenium by HG-AFS was proposed in this paper. The experimental conditions were optimized and the influence of co-exist foreign ions was investigated. The proposed method was successfully applied to the determination of selenium in ten kinds of Chinese herbs.In chapter 4, a method, hydride generation atomic fluorescence spectrometry, for the determination of arsenic and mercury in traditional Chinese herbs. The experimental conditions and the instrumental operation parameters were optimized. The interference of coexistent elements in traditional Chinese herbs for the determination of As and Hg were investigated. The accuracy of the method was confirmed by the standard addition method. This method was proved to be simple, rapid and repeatable.更多还原