【作者】 李桂花；

【导师】 刘新祥；

【作者基本信息】 湖南中医学院 ， 中医内科， 2003， 硕士

【摘要】 目的：探讨肾安冲剂治疗肾病综合征的作用机制方法：采用阿霉素（ADR）尾静脉注射制造肾病综合征大鼠模型。将实验大鼠随机分为肾安冲剂组、强的松组、肾安冲剂+强的松组、模型组和正常对照组。各组均从造模后2周开始给药，连续8周。取标本进行24小时尿蛋白定量、血清白蛋白（Alb）、血尿素氮（BUN）、血肌酐（Scr）、血脂、脂蛋白脂酶（LPL）、肾脏病理形态学及Ⅰ、Ⅲ型胶原的检测。结果：1. 与正常组比较,模型组大鼠24小时尿蛋白定量、血脂水平明显升高,血清白蛋白水平、脂蛋白脂酶活性明显降低,肾小球系膜细胞个数、系膜基质面积和Ⅰ、Ⅲ型胶原面积比均明显增加(均p<0.01). 2.与模型组比较,各治疗组大鼠24小时尿蛋白定量、血脂水平明显降低,血清白蛋白水平、脂蛋白脂酶活性明显升高,肾小球系膜细胞个数、系膜基质面积和Ⅰ、Ⅲ型胶原面积比均明显减少(p<0.05或p<0.01)。3.肾安冲剂组各检测指标与强的松组比较均有显著性差异(p<0.05或p<0.01),说明肾安冲剂疗效优于强的松。结论：肾安冲剂能够明显降低24小时尿蛋白定量,升高Alb水平,提高LPL活性,降低血脂水平,抑制肾小球系膜细胞、系膜基质的增生,减少Ⅰ、Ⅲ型胶原的沉积,从而减少了各病理因素的肾损害,最终抑制了ADR肾病大鼠肾组织细胞外基质（ECM）的增生和积聚，延缓了肾小球硬化和肾间质纤维化的进程。更多还原

【Abstract】 Objective: To study the therapeutic mechanism of SACJ treating nephritic syndrome.Methods: Nephrotic syndrome(NS) rat models were induced by tail intravenous injection with adriamycin(ADR). The experiment rats were randomly divided into SACJ group, Prednison group, SACJ combined with Prednison group, the model group and normal control group. 2 weeks after the models being made, the admistration of drugs started and lasted for 8 weeks. The content of 24- hour urinary protein, serum albumin, (Alb), BUN, Scr , blood lipid level, activities of lipoprotein lipase (LPL),morphology of renal histology, collagen I and collagen Ⅲ were detected . Results: 1.The contents of 24-hour urinary protein, lipid level increased and serum albumin level ,activities of LPL decreased significantly in model rats compared with normal rats (p<0.01).The number of mesangial cell, the area of mesangial matrix and the surface area rate of collagenⅠand collagen Ⅲ of model rats were obviously higher than those of normal rats(p<0.01).2. The contents of 24-hour urinary protein and lipid level of all treated group rats were obviously lower than those<WP=5>of model group rats (P <0.05or p<0.01). Serum albumin level and activities of LPL of all treated group rats were obviously higher than those of model group rats (p<0.05 or p<0.01).The number of mesangial cell, the area of mesangial matrix and the surface area rate of collagenⅠand collagen Ⅲ were significantly reduced in all treated group rats compared with model group rats (p<0.05 or p<0.01).3.There were markedly differences of all test parameters between SACJ group and Prednison group which indicated the therapeutic effect of SACJ was better than that of Prednison for nephritic syndrome. Conclusion: SACJ decreased the contents of 24-hour urinary protein and lipid level, increased Alb level and activities of LPL, inhibited the proliferation of mesangial matrix and mesangial cell and reduced the deposition of collagen Ⅰ and collagen Ⅲ.So it relieved the lesion of kidney owing to the pathologic agents. SACJ could fundamentally inhibit the ECM accumulation of ADR rats and slow the processes of glomerulosclerosis and renal interstitial fibrosis更多还原