【作者】 康孟佼；

【导师】 朱国强； 王永坤；

【作者基本信息】 扬州大学 ， 预防兽医学， 2003， 硕士

【摘要】 2001年江苏省某养殖场鹅群中爆发了一种新的传染病，该病只引起幼龄鹅发病，死亡率在70％左右，死亡鹅以肝脏出血坏死为主要的病理变化，故我们将其命名为鹅出血性坏死性肝炎。 将发病鹅肝脏无菌研磨液通过肌肉注射接种动物，复制到了与自然发病基本一致的临床症状和病理变化；并制备了发病鹅肝脏、脾脏、肾脏的病理切片，对其微观病变进行了观察。用离子捕捉法对肝脏无菌研磨液进行处理，经过负染和电镜观察，发现病毒粒子呈球型，双层衣壳，外层衣壳直径约75nm，内层约50nm，无囊膜；该病毒粒子对乙醚、氯仿、胰蛋白酶有抵抗作用，耐酸、耐热；病毒不能通过尿囊腔途径生长，通过尿囊膜接种适日龄鸡胚、鸭胚、鹅胚，一周内有80％左右的死亡率；病毒在鸡胚成纤维细胞上盲传5代后，能够诱发鸡胚成纤维细胞出现稳定的病理变化，病理变化主要是细胞融合和合胞体的出现。对病毒将要致死鸡胚器官取材；按时间梯度，对接种病毒的鸡胚成纤维细胞连续取材，分别制成超薄切片在电镜下观察，发现病毒粒子在胞浆中复制，不成熟病毒粒子有时形成包涵体，对病毒所造成的组织损伤也进行了描述。 依照国际病毒委员会病毒分类原则，参考该病毒的性质，初步鉴定该病毒为呼肠孤病毒科正呼肠孤病毒属禽呼肠孤病毒。更多还原

【Abstract】 A new disease was out breaking in the geese no older than one month in JiangSu province with 70% mortality. We named the disease goose hemorrhagic necrotic hepatitis according to its mainly pathologic changes.Replicate the disease by inoculating goose the suspension of liver; the desired disease appeared 7 days later, which was consistent with the nature induced one. The liver, spleen and kidney of goose were made out pathologic slices. The virus in the liver suspension dealing with the technology of ion exchange capture showed spherical, no envelope, having double-deck capsid, which range from 75nm-50nm in diameter by TEM. The virus resist to aether, chloroform,typton,heat and acid; the virus give the chicken embryo, duck embryo, goose embryo a 80% mortality inoculating by the way of allantochorion and can’t adapt to allantoic cavity. The virus, after continuously propagating in chicken embryo fibroblast for 5 generations, will induce the CEF stable pathogenesis such as fusion cell. The ultrathin sections which were made out from the infected CEF at progressive time and from the chicken embryo infecting 72h later were observed by TEM, the results suggested that the virus exist by single or endobody and replicate in cytoplasm.According to the ICTV principles, all the information above indicate this virus reoviridae ,orthoreovirus,avian reovirus.更多还原