中医阳黄证黄疸动物模型的建立及中药退黄对模型影响的实验研究

【作者】 王文丽；

【导师】 曲长江；

【作者基本信息】 辽宁中医学院 ， 中医基础理论， 2003， 硕士

【摘要】 目的：建立中医阳黄证黄疸动物模型。方法：在采用现代医学异硫氢酸苯酯(APIT)灌胃诱导大鼠肝损伤的基础上，结合中医高脂高糖饮食加湿热环境因素，建立中医阳黄证黄疸动物模型。观察大鼠一般生物学状态、肝功能变化及体液免疫水平，并予中药复健方茵陈蒿汤治疗。从病因、症状、体征、实验指标以及中药治疗反证等方面对模型加以评价。结果：中医阳黄证黄疸动物模型符合黄疸证疾病特点，未改变黄疸模型的关键指标，未影响异硫氢酸苯酯(APIT)灌胃造模方法的主要特点；同时模型大鼠出现明显湿热证主证及湿热证客观指标变化，经中药复健方治疗后，黄疸证指标和湿热证主证及指标明显改善。结论：采用现代医学异硫氢酸苯酯(APIT)灌胃，结合中医高脂高糖饮食加湿热环境因素建立的中医阳黄证黄疸动物模型符合中医学传统病因病机理论，方法可靠，重现性强，与人类阳黄证相似，为中医药研究治疗黄疸阳黄证提供了较为合适的动物模型。更多还原

【Abstract】 Purpose: To establish an animal model of TCM jaundice of Yang-Huang. Materials and Methods: We got the animal .model of TCM jaundice of Yang-Huang in rats, which lived in the damp and hot environment and feed on high-fat-sucrose diet , with the injury of liver induced by oral APIT. We observe the general biological state, liver function, humoral immunity, and the changes after treating with Chinese herbal decoction .("Yinchenhao " decoction) in rat. The model was evaluated with etiologic factor, sumptom, sign, experimental data, and the disproof of treating with herbal decoction.Result: The model of TCM jaundice of Yang-Huang in rat fit the character of jaundice, which didn’t change key point marke of jaundice model, didn’t effect 昪hief behavior of APIT jaundice model. The rats of the model all have the symptoms of dampness-heat syndrome and have the significant changes in experimental data of dampness-heat syndrome. The experimental data of jaundice and dampness-heat syndrome are both improved significantly treating with herbal decoction.Conclusion: The animal model of TCM jaundice of Yang-Huang in rats, which lived in the damp and hot environment and feed on ? , with the injury of liver induced by oral APIT is in accord with the TCM theory in etiology and pathology and similar with the clinical symptom in human. It is suitable and potentially useful in the study of TCM treatment of jaundice.更多还原