【作者】 米彦；

【导师】 孙才新；

【作者基本信息】 重庆大学 ， 电气工程， 2003， 硕士

【摘要】 为了研究能量可控陡脉冲对恶性肿瘤细胞不可逆性电击穿的机理，并将能量可控陡脉冲疗法应用于恶性肿瘤的临床治疗，本论文研制出了一套能对输出陡脉冲的能量进行精确控制的陡脉冲发生装置，并应用该装置进行了大量的医学细胞实验和动物实验。首先，将传统脉冲功率技术中陡脉冲产生的基本原理和现代电力电子技术相结合，研制成功可控陡脉冲装置。通过开关式稳压电源产生高压直流，并控制该电压对电容器进行充放电就能输出指数衰减陡脉冲波形。利用工控机分别调节开关式稳压电源控制电路和电容充放电控制电路中的数字电位器，就能分别调节输出陡脉冲的幅值和重复频率；利用工控机控制继电器选择不同的充放电电容，就能调节输出陡脉冲的脉宽。通过调节输出陡脉冲的峰值、重复频率和脉宽，就能对输出陡脉冲的能量进行精确控制。然后，应用该装置对人卵巢腺SKOV3癌细胞进行了大量的细胞实验。实验结果表明，陡脉冲能有效杀伤恶性肿瘤细胞，它通过在细胞周期中抑制DNA的合成来抑制细胞的增殖。最后，应用该装置对接种宫颈鳞癌的荷瘤BALB/C小鼠进行了大量的动物实验。实验结果表明，陡脉冲能有效地杀伤肿瘤细胞，明显抑制肿瘤组织的生长，延长荷瘤小鼠的生存期。实验室的调试结果和医学实验结果表明，可控陡脉冲装置具有非常优越的性能，完全满足了医学实验的需要，达到了预期的目标，为能量可控陡脉冲疗法应用于恶性肿瘤的临床治疗打下了坚实的基础。同时，这些医学实验结果也表明，能量可控陡脉冲疗法有着非常乐观的应用前景。更多还原

【Abstract】 Combining High Voltage New Technology and Power Electronics Technology, this paper developed a Controllable Steep Pulse Generator (CSPG) that can control the energy of output pulse accurately. Applied with this generator, a lot of cell experiments and animal experiments were carried out. Based on these works, it’s easier to research mechanism of Irreversible Electrical Breakdown (IREB) of malignant tumor cell under steep pulse, and it may be helpful to generalize Energy Controllable Steep Pulse Therapy (ECSPT) on cancer treatment.At first, produce direct current high voltage by Switching Power Supply (SPS). Then, control this voltage to charge a capacitor through a resistor. Discharge the capacitor to a load after it has charged fully enough, and this produces an Exponential Decay Pulse (EDP) on the load. Adjusting digital potentiometer in controlling circuits of SPS and capacitor charging and discharging circuit separately with Industry Control Computer (ICC), the peak value and repeat frequency of output pulse can be adjusted separately. Controlling relays to choose different capacitors with ICC, the width of output pulse can be adjusted.Then, applied with CSPG, cell experiments on human’s ovarian adenocarcinoma SKOV3 cells were carried out. The result shows that steep pulse can kill and wound malignant tumor cell effectively. It can restrain DNA synthesis in cell cycle, so cancer cell multiplication is restrained. Finally, applied with CSPG, animal experiments on BALB/C mice inoculated with cervical squama cancer were carried out. The result shows that steep pulse can kill and wound malignant tumor cell effectively. It can restrain the growth of tumor tissue and prolong survival time of the mice obviously. The laboratory debugging result and medical experiments results both show that CSPG has excellent performance, and it satisfied the needs of medical experiments fully and got the expected goal successfully. This provides the foundation for applying ECSPT on clinical cancer treatment. In addition, these medical experiments results also show that the future of ECSPT is quite optimistic.更多还原