【作者】 张玉倩；

【导师】 王廷华；

【作者基本信息】 昆明医学院 ， 人体解剖与组织胚胎学， 2003， 硕士

【摘要】 目的：采用免疫组织化学和原位杂交技术，探讨针刺对备用背根节卫星细胞NGF，BDNF，NT-3表达的影响。方法：成年猫5只，行双侧部分背根切断术（切除两侧L₁-L₅，L₇-S₂ DRG，保留L₆为备用根）。术后当日开始针刺一侧L₆脊神经后肢分布区的两组穴位即足三里和悬钟、伏兔和三阴交，每天一次，每次30分钟。术后7天，取非针刺侧和针刺侧的L₆ DRG制作20μm厚冰冻切片，行免疫组化及原位杂交染色。观察NGF、BDNF和NT-3免疫阳性反应（IR）在DRG卫星细胞的分布，测量各组L₆ DRG卫星细胞NGF、BDNF和NT-3的光密度值，结果用q检验进行统计分析。结果：在DRG，可见NGF、BDNF和NT-3及其mRNA阳性卫星细胞。各因子mRNA的阳性反应物定位于胞质；BDNF-IR也定位于胞质，而NGF-IR和NT-3-IR则定位于胞核。卫星细胞NGF、BDNF、NT-3及其mRNA阳性反应的光密度值针刺侧较非针刺侧明显增多（P＜0.05）。结论：背根节部分卫星细胞内有NGF、BDNF和NT-3及其mRNA表达；针刺导致备用DRG卫星细胞NGF、BDNF和NT-3表达上调。结果提示：背根节卫星细胞内NGF、BDNF和NT-3的表达变化可能与针刺促进脊髓可塑性有关。更多还原

【Abstract】 Objective To investigate the effect of acupuncture on the expression of NGF, BDNF, NT-3 and their mRNA in satellite cells of dorsal root ganglion (DRG) in adult cat following partial dorsal root rhizotomy. Methods Five adult cats underwent partial bilateral root rhizotomy (L1-L5, L7-S2 DRG were taken and L6 DRG was spared), The cats received acupuncture for 7 days following operation (The acupuncture points including Zusanli and Xuanzhong, Futu and Sanyinjiao located in the distribution area of L6 spinal nerve in one side of hind limb were stimulated alternatively for 30min every day by electro-needle). The L6 DRGs from acupuncture side and unacupuncture side were taken and made into frozen section 20μm in thickness respectively. The sections were stained by immunohistochemistry ABC method and in situ hybridization technique. The distribution of positive satellite cells for NGF, BDNF, NT3 was observed under microscope, The 0. D value of positive satellite cells for NGF, BDNF and NT3 was measured and analyzed statisticallyby q-test. Results NGF, BDNF, NT-3 and their mRNA were seen in some satellite cells of DRG. The positive signal of mRNA for NGF, BDNF and NT3 was observed in cytoplasm , BDNF-IR was located in cytoplasm, while the NGF-IR and NT3-IR were distributed in nucleus.The O.D value of satellite cells for NGF, BDNF and NT3 after operation following acupuncture increased apparently more than that of operation without acupuncture. Conclussion: NGF, BDNF and NT3 are expressed in satellite cells of DRG. And the expression for three factors was upregulated in satellite cells following acupuncture.The results indicated that the change of NGF, BDNF and NT3 in satellite cells of DRG may be involved in the plasticity of spinal cord which is promoted by acupuncture.更多还原