【作者】 李强；

【导师】 兰利琼； 傅华龙；

【作者基本信息】 四川大学 ， 植物学， 2003， 硕士

【摘要】 本文通过在培养基中加入不同比例的NAA与6-BA对产生胚状体和鳞茎的影响实验，发现当NAA和6-BA分别为2mg／L和1mg／L，蔗糖为30g／L时愈伤组织上胚状体生成个数最多，平均每个愈伤组织上生有20个左右的胚状体，愈伤组织生长迅速，因而此浓度可用于川贝母愈伤组织诱导胚状体；同时，当NAA和6-BA分别为1mg／L和0.5mg／L，蔗糖为20g／L时愈伤组织上鳞茎生成个数最多，鳞茎色泽为乳白色，且愈伤组织的生长率最大，因而此比例的激素配比最有利于鳞茎的诱导。 在组培川贝母(Fritillaria Cirrhosa D.Don)鳞茎形成和发育过程中，获得愈伤组织、胚性愈伤组织、胚状体、小鳞茎、大鳞茎。分别作过氧化物酶、过氧化氢酶、淀粉酶电泳分析和活性分析。结果表明，3种酶都参与了川贝母鳞茎形成和发育，且酶的表达量和活性随不同的时间发生变化。过氧化物酶的B区酶带变化明显，小鳞茎阶段活性较高；过氧化氢酶在胚状体和大鳞茎阶段几乎不表达，但在小鳞茎阶段表达量增加十分明显，活性也较高；淀粉酶的3条谱带在胚状体阶段表达明显活性也最高，但在大鳞茎阶段则几乎没有表达。 组培川贝母(Fritillaria Cirrhosa D. Don)的胚状体经低温处理不同的时间，随后转入无激素的MS培养基中培养，发现低温处理40d的胚状体成苗率最高。对过氧化物酶的活性及其电泳图谱的研究发现，在低温处理及随后的培养过程中，过氧化物酶的活性、表达量及谱带数(特别是B区酶带)发生明显的变化。C区酶带的出现，是川贝母低温处理诱导胚状体成苗的一个重要因素。更多还原

【Abstract】 In this paper,induction of embryoid and bulb of Fritillaria.D.Don from callus were studied.The result has indicated that in case of adding NAA 2mg/L,6-BA Img/L and sucrose 30g/L to the MS medium,and the number of the induced embryoid was the most; in case of adding NAA lmg/L,6-BA 0.5mg/L and sucrose 20g/L to the MS medium,the number of the differentiated bulb was the most.Plant issue culture method of Fritillarir Cirrhosa D.Don was established. Author analysed peroxide,scatalase and amylase by electrophoresis and studied their activites during bulb organ genesis and development and found that in the course of bulb organ genesis and development there were various isozymes which took part in it together. B region of peroxide changed obviously. In small bulb its activity was the highest Scatalase did not express in embryoid and big bulb, but express significantly in small bulb compared to the others, and its activity was also the highest. Amylase expressed obviously in embryoid, and its activity was also the highest ,but expressed hardly in big bulb.After embryoids were cultured at a low temperature of 0-5 D for 0 to 60days,the rate of germination and several physiological characters were studied. Theauthor found that the number of the further germination of embryoids was more in40 days treatment than others on MS medium of no hormones.Furthermore,theauthor analyzed peroxide by electrophores- is,and found that embryoids occuredapparent changes of activity and map bands,especially in B region and C region.更多还原