【作者】 杨恩月；

【导师】 洪淳赞；

【作者基本信息】 延边大学 ， 药理学， 2003， 硕士

【摘要】 目的：研究蓝靛果乙酸乙酯提取物（Ethyl acetate extraction of Lonicera caerulea L.Var.edulis Turcz. ex Herd, EELC）对S180和H22荷瘤小鼠抑瘤作用及其作用机制。 方法：用EELC灌胃给小鼠观察其对荷瘤小鼠肿瘤的抑制作用，检测免疫器官重量；用比色法测荷瘤小鼠血清超氧化物歧化酶(superoxidedismutase,SOD)、一氧化氮合酶(nitric oxide synthase,NOS)的活性及丙二醛(malondialdehyde,MDA)、一氧化氮(Nitricoxide,NO)、血清唾液酸（Sialic acid,SA）的含量；用放射免疫法测血清肿瘤坏死因子（tumor necrosis factor ,TNF-α）的含量；取瘤组织块，甲醛固定，切片，H·E染色，图象分析仪400倍光镜视野观察核分裂相数及超低倍镜下观察瘤组织内坏死程度。观察小鼠H22腹水瘤的存活情况。 结果：EELC对S180和H22小鼠实体瘤有明显的抑制作用，EELC大、中、小剂量组对S180的抑瘤率分别为50.99%、39.45%、27.84%，对H22的抑瘤率为30.09%、19.16%、13.33%；能显著提高S180和H22小鼠的胸腺指数，但对脾指数无明显作用；并能明显提高S180小鼠血清SOD的活性，降低NOS的活力，同时能显著降低MDA、SA、TNF-α及NO的含量；提高巨噬细胞的吞噬功能；病理形态学上，能抑制瘤细胞分裂增殖,可使瘤组织大片坏死；能明显降低H22小鼠血清NOS的活力和SA的含量，同时对H22腹水瘤小鼠有一定的提高生命质量的作用。结论 ：EELC对S180和H22荷瘤<WP=4>小鼠有明显的抑瘤作用，提高生命质量，其可能机制是通过提高抗氧化活性，清除自由基，改善小鼠免疫功能，抑制肿瘤细胞增殖以及使肿瘤组织大片坏死而实现的。更多还原

【Abstract】 OBJECTIVE: To investigate the tumor-inhibiting action Ethyl acetate extraction of Lonicera caerulea L. Var. edulis Turcz. Ex Herd (EELC) on mice bearing sarcoma 180(S180) and hepatocarcinoma 22(H22), and its therapeutic mechanism. METHOD: EELC was administered orally EELC to mice, and tumor inhibiting action on tumor-bearing rat was observed. Immune organs were weighed; the activity of superoxidedismutase (SOD), nitric oxide synthase (NOS) and the content of malondialdehyde (MDA), Nitricoxide(NO), Sialic acid(SA) were detected by colorimetry; and the content of tumor necrosis factor (TNF-α) by Radioimmunoassay; pieces of tumor was adopted, fixated by formaldehyde, sliced up and H·E colorated. We used the image analyzer to observe the numbers segmentation of nucleus with 10 high power field of light mirror,and observed the necrosis degree of Neoplasia constitution with the super low power field of light mirror. And during the test we recorded the stock condition of mice bearing H22 hydroperitonia Neoplasia. RESULTS: EELC inhibited the growth of solid tumor of mice bearing S180 and H22 obviously. In the group of high, middle and low dosage, the tumor inhibition rate on mice bearing S180 were 50.99%, 39.45%and 27.84% respectively, more over the tumor inhibition rate on mice bearing H22 were 30.09%, 19.16% and 13.33% respectively;<WP=6>could increase the thymus exponent of mice bearing S180 and H22, but had no effect on the spleen exponent; EELC could increase the SOD activity in mice bearing S180 blood serum and inhibit the activity of NOS, moreover decrease the content of MDA, SA, TNF-αand NO.; increase the phago function of macrophages; in pathomorphology，could inhibited the cell division, proliferation, and promoting the death of the tumor cells; could decreased the the activity of NOS and content of SA in mice bearing H22 blood serum significantly; and could prolong the life time of mice bearing H22 hydroperitonia Neoplasia. CONCLUSION: EELC inhibited growth of tumor significantly, enhanced the immune functions and life quality of mice. The possible mechanism is that: EELC might inhibite the proliferation of tumor cells and accelerate the necrosis of tumor tissues through improving the ability of body anti-oxidation activity, clearing free radicals and increasing the immune functions.更多还原