【作者】 詹自力；

【导师】 苟欣；

【作者基本信息】 重庆医科大学 ， 外科学， 2003， 硕士

【摘要】 目的 ：研究P27、cyclin D1和cyclin E蛋白在膀胱移行细胞癌中的表达及意义。方法： 采用免疫组织化学方法检测65例膀胱移行细胞癌的石蜡标本、10例膀胱粘膜非典型增生、8例正常膀胱组织中P27、cyclin D1和cyclin E的表达。结果： 正常膀胱组织p27阳性表达率为100%，非典型增生标本中p27阳性表达率为60%，低于正常膀胱组织(P<0.05)；膀胱癌组织中p27阳性表达率仅为51%，明显低于正常膀胱组织(P<0.01)。p27阳性表达率在G1、G2、G3膀胱癌中分别为75%，53.8%，26.3%，三组间相比较，差别均有显著性意义(P<0.05)。G1组与G3组比较有显著性意义。Ta-T1、T2-T4两组中，p27的阳性表达率分别为72.9%、25%，两组比较有显著性意义(P<0.01)。 cyclin E在非典型增生组织中阳性表达率为60%，明显高于正常膀胱组织的25%，但统计学上无显著性意义。在膀胱癌G1、G2、G3三组中cyclin E的阳性表达率分别为80%、42.3%、31.6%，三组比较有显著性意义(X2=10.38 P<0.01)，其中G1组cyclin E的阳性表达率明显高于G2、G3组(P<0.01)。在Ta-T1、T2-T4两组中，cyclin E的阳性表达率分别为<WP=6>62.2%、35.7%，两组比较有显著性意义(P<0.05)。cyclin D1在正常膀胱组织中没有表达，在非典型增生组织中阳性表达率为40%，在肿瘤组织中cyclin D1阳性表达率为38.5%(P<0.05)，两者明显高于正常组织。在G1、G2、G3组膀胱癌中cyclin D1阳性表达率分别为65%、34.6%、18.6%，三组间相比较有显著性意义(P<0.01)。G1组cyclin D1的阳性表达率明显高于G2、G3组(P<0.01)。 在Ta-T1、T2-T4两组中，cyclin D1的阳性表达率分54.1%、17.5%，两组比较有显著性意义(P<0.01)。P27、cyclin D1和cyclin E两两相关性分析显示呈正相关。结论：本试验表明：P27蛋白表达下降或缺失及cyclin D1和cyclin E过表达可能加速细胞周期转化，参与膀胱癌的发生。cyclin D1过表达可能是膀胱癌形成过程中较早的分子事件。随着膀胱移行细胞癌的病理分级、临床分期增加，P27、cyclin D1和cyclin E而呈下降趋势。检测P27、cyclin D1和cyclin E在膀胱癌组织中的表达，有助于估计肿瘤的恶性程度和判断患者的预后。更多还原

【Abstract】 Objective To study the expression and it’s significance of P27,cyclin D1 and cyclin E protein in transitional cell carcinoma of bladder(TCCB).Methods Expression of P27 cyclin D1 and cyclin E protein were investigated by immunohistochemistry technique (SP) with 65 cases of transitional cell carcinoma of the bladder, 10 atypical hyperplasia cases and 8 normal bladder cases.Results The postive immunostaining rate of P27 in normal bladder (100%) was significantly higher than in the atypical hyperplasia and significantly higher than in the TCCB (51%). The postive immunostaining rate of P27 was 75% of G1, 53.8% of G2, 26.3% of G3,72.9% of Ta-T1 and 25% of T2-T4.There was significant difference either among G1,G2,G3(P<0.05) or between Ta-T1 and T2-T4(P<0.01). cyclin E in the atypical hyperplasia(60%) was insignificantly higher than in the normal bladder tissue(25%). The expression rate of cyclin E was 80% of G1,42.3% of G2,31.6% of G3,62.2% of Ta-T1 and 35.7% of T2-T4. There was significant difference either among G1, G2, G3 (P<0.05) or between Ta-T1 and T2-T4 (P<0.01). The expression rate of<WP=8>G1 was significantly higher than G2 and G3, The expression of cyclin D1 in the normal bladder tissue was negative. The expression rate of cyclin D1 in TCCB (38.5%) and in the atypical hyperplasia(40%)was significantly higher than that in the normal bladder tissue. The expression rate of cyclin D1 was 65% of G1, 34.6% of G2, 18.6% of G3,54.1% of Ta-T1 and 17.5% of T2-T4. There was significant difference either among G1, G2, G3 (P<0.01) or between Ta-T1 and T2-T4 (P<0.01). The expression rate of cyclin D1 of G1 was significantly higher than that of G2 and G3. P27, cyclin D1 and cyclin E were correlated with each other. Conclusions These findings suggest that the down or negative-expression of P27 and overexpression of cyclin D1 and cyclin E might accelerate the progression of the cell cycle and promote the carcinogenesis of the TCCB.The overexpression of cyclin D1 might be an relatively earlier event in the initition of the TCCB. With malignance of tumor increasing, the expression rate of them was decreasing. Analysis of P27、cyclin D1 and cyclin E expression in TCCB was useful for us to estimate the malignance and prognosis.更多还原