【作者】 戴开金；

【导师】 罗佳波；

【作者基本信息】 中国人民解放军第一军医大学 ， 中药药剂学， 2003， 硕士

【摘要】 葛根芩连方药味简单，配伍精当，药理作用、化学成分研究有较好的基础。选用此方研究配伍对化学成分的影响，具有一定的代表意义。 目的：建立葛根芩连方中有效成分含量及指纹图谱分析方法，研究配伍对有效成分含量及指纹图谱的影响。 方法：样品用醇水处理后，采用了HPLC法测定了该方各种配伍煎液中有效成分葛根素、黄芩甙、小檗碱、甘草酸的含量；采用HPCE法测定了配伍对该方指纹图谱的影响；采用正交设计及统计分析方法对结果进行分析。 结果：(1)分析方法简单，有较好的专属性和重现性。 (2)黄连、黄芩、甘草对葛根素的含量及指纹图谱中葛根G1、G2、G3、G5、G6峰峰面积的影响，没有显著性差异(p＞0.05)。 (3)葛根、黄芩都降低黄连中小檗碱的含量和指纹图谱中黄连L5峰的面积(p＜0.05)，葛根、黄芩的交互作用对指纹图谱中黄连L5峰的面积存在显著性差异(p＜0.05)，葛根、黄芩对黄连L1、L4峰面积的影响，差异不显著(p＞0.05)；甘草降低黄连中小檗碱的含量，对指纹图谱中黄连L1、L4、L5峰面积的影响，差异不显著(p＞0.05)。 (4)葛根降低了黄芩中黄芩甙的含量(p＜0.05)，对指纹图谱中黄芩Q1、Q2、Q3、Q4峰面积的影响，差异不显著(p＞0.05)；黄连降低了黄芩中黄芩甙的含量及指纹图谱中黄芩Q4峰的面积；甘草对黄芩中黄芩甙的含量及指纹图谱中黄芩Q1、Q2、Q3、Q4峰峰面积的影响，差异不显著(p＞0.05)。 (5)黄连降低甘草中甘草酸的含量(p＜0.05)，黄芩、葛根对甘草酸含量影响，没有显著性差异(p＞0.05)。 (6)黄连与黄芩、葛根、甘草合煎时都会有沉淀产生，沉淀中分别含有小檗碱、黄芩甙、葛根素、甘草酸等物质，其中甘草酸基本完全沉淀，葛根素在沉淀中的含量较少，沉淀的主要成分为黄芩甙和小檗碱。 结论：配伍对方中有效成分及指纹图谱有一定的影响，含黄连的配伍组合产生了沉淀并使有些成分含量降低的主要原因是煎液pH值的改变。配伍降低了黄连中小檗碱的含量，为黄连较少单独使用提供物质基础上的理论依据。更多还原

【Abstract】 Gengeng-Qinlian Decoction(GQD) is a famous prescription by extraordinarily skilled practitioner Zhangzhongjing, which is composed of Pueraria lobata Ohwi, Coptis chinensis Franch, Scutellaria baicalensis Georgi and Glycyrrhiza uralensis Fisch. It can relieve exterior syndrome, remove obstruction due to interior syndrome, ascend the clear, stop diarrhea and has been widely used in clinical routine. But to GQD, the effective assay methods is lack and the study on compatibility is deficiency too.Objective: To develop the assay methods of the effective components(EC) and the fingerprint (FP) of GQD. To study the influence of compatibility on the content of EC and FP.Method: After a simple sample extraction with methanol solution, high-performance liquid chromarography(HPLC) was adopted to assay the content of EC and high -performance capillary electrophoresis(HPCE) was adopted to assay FP. Orthogonal design was used in this research and the data was analyzed with statistics analysis(SPSS 10.0).Result: (1) .All these methods were simple, accurate and reproducible. (2) For Scutellaria baicalensis Georgi and Glycyrrhiza uralensis Fisch, the difference on the content of kakonein, and the difference on the peak area of the Pueraria lobata Ohwi’s G1, G2, G3, G5, G6 peak in FP were insignificant(P>0.05);(3) For Pueraria lobata Ohwi and Scutellaria baicalensis Georgi , they may reduce the content of berberine and the peak area of the Coptis chinensis Franch’s L5 peak in FP(P<0.05); the difference on the peak area of the Coptis chinensis Franch’s L5 peak in FP was significant(P<0.05) , but the difference on the peak area of the Coptis chinensis Franch’s L1 and L4 peak in FP was insignificant(P>0.05). For Glycyrrhiza uralensis Fisch , it may reduce the content of berberine, and the difference on the peak area of Coptis chinensis Franch’s L1,L4 and L5 peak in FP were insignificant(P>0.05).(4) For Pueraria lobata Ohwi, it may reduce the content of baicalin(P<0.05), and the difference on the peak area of Scutellaria baicalensis Georgi’s Q1, Q2, Q3 and Q4 peak in FP were insignificant(P>0.05).For Coptis chinensis Franch,it may reduce the content of baicalin and the peak area of the Scutellariabaicalensis Georgi’s Q4 peak in FP(P<0.05). For Glycyrrhiza uralensis Fisch,the difference on the peak area of Scutellaria baicalensis Georgi’s Q1, Q2, Q3and Q4 peak in FP were insignificant(P>0.05).(5)For Coptis chinensis Franch, it may reduce the content of glycyrrhiza . ForScutellaria baicalensis Georgi and Pueraria lobata Ohwi, the difference onthe content of glycyrrhiza was insignificant(P>0.05).(6) When Coptis chinensis Franch decocted with Scutellaria baicalensisGeorgi, Pueraria lobata Ohwi or(and) Glycyrrhiza uralensis Fisch, thesedimentation appeared, berberine, baicalin, kakonein and glycyrrhiza werefound in it, it was proved that the main component were berberine andbaicalin.Conclusion: The the content of EC and the FP of GQD.were influenced bycompatibility. Sedinentation appeared when Coptis chinensis Franch wasincluded in the compatibility, the main reason is the change of pH leads tosedimentation in decoction and reducing of some components.更多还原