【作者】 杨志兵；

【导师】 陈进；

【作者基本信息】 武汉理工大学 ， 控制理论与控制工程， 2003， 硕士

【摘要】 微生物发酵是许多行业的一个关键生产过程，真菌又是食品和药物方面最具潜力的发酵微生物种类之一。真菌发酵应用于有用代谢产物的生产具有很多优点，因此必将成为未来获得大量真菌有用代谢物的一种重要方法。溶解氧问题广泛存在于众多学科，对溶解氧进行有效控制有着重要的科学和工程意义，然而到目前为止，微生物发酵过程溶解氧的自动控制问题还没有得到真正解决。工业发酵生产要求对发酵过程进行优化和控制。本论文主要讨论了真菌深层发酵生产γ-亚麻酸的过程中溶解氧的自动控制问题，设计了真菌深层发酵自动控制系统并建立了基于泛布尔代数的溶解氧自动控制模型。全文共分五章。 首先简要介绍了目前微生物发酵过程自动控制的热点研究问题和溶解氧自动控制的研究现状，并对溶解氧相关问题及溶解氧控制问题作了简要说明。 对发酵的过程参数进行在线监测及数据采集是对整个发酵过程进行监控和研究的必备条件。在第二章中，本文对目前微生物发酵过程参数的检测状况进行了全面的介绍，主要包括对发酵过程中溶解氧和其它各参数进行测量的常用方法和仪表，以及发酵过程对这些方法和仪表的特殊要求。 第三章主要围绕微生物深层发酵过程控制系统的设计与实现展开。根据发酵罐的特点，本文基于单片机和PC机结合，设计了发酵过程自动控制系统，实现DO、pH、温度、罐压、通气量和搅拌电机转速的测控以及发酵液的粘度的在线测量。对系统的硬件和软机构成和工作原理以及对各种发酵过程参数的检测和控制方法作了详细说明。 第四章对发酵过程中溶解氧的影响因素作了全面的综述。在分析了大量实验数据的基础上，针对微生物深层发酵过程中非线性、大滞后、时变的溶解氧系统，建立了基于泛布尔代数的逻辑控制模型。此方法根据实验经验和逻辑控制规则，不依赖于被控对象的精确数学模型，避免了常规控制算法建立对象精确数学模型的困难。本文随后设计了该逻辑算法的仿真实验，验证了算法的可行性。结果表明，与传统的控制算法相比，该方法还在稳定性方面具有突出的优点。 最后，对本文所做的工作以及逻辑控制算法进行了简短的总结。更多还原

【Abstract】 For many industries, fermentation is a fundamental process, and eumycete is one of the most promising fermenting microbes for food and medicine. It is much advantageous to get secondary metabolin by eumycete fermentation, and will be a important way to produce the metabolin. Dissolved Oxygen (DO) is involved in many domains of science, and it has great science and engineering significance to apply control over DO. However, DO control in microbe fermentation process is indeed left unsolved so far. Industrial fermentation requires optimized and controlled condition. This dissertation mainly deals with the control over DO in microbe fermentation process producing y-linolenic, devises a control system and builds up a DO controlling model based on Pan-bool algebra. The whole work consists of five chapters.Firstly, this paper outlines noticeable researches on microbe fermentation auto control , DO involves and DO control at present.To fermentation process, to measure and collect the data of the parameters is the premise of the control over them. Chapter 2 argues the present status of parameter measurement in fermentation, including the methods and equipment, and the restrictions fermenter applies to them.Chapter 3 primarily informs the fermentation process control system devising and the structure of the system. According to the characteristic of fermenter, we designed the control system based on single chip computer and personal computer, realizing the measurement and control over DO, pH, temperature, pressure, ventilating rate and the speed of the stirring motor. The paper details the hardware and software structure, and the working principle of the system, as well as the method to measure and control the parameters.Chapter 4 discusses the factors influencing DO in the process of fermentation in detail. Analyzing much experiment result, the paper presents a logical controlling method based on Pan-bool algebra and constructs the controlling model in allusion to the non-linear, time-varying, delaying DO control system. This theory depends on experience and logical control laws and needs no accurate mathematic model of the object, so approaches the difficulties traditional control method encounters building accurate model of the object. By simulation, the papersubsequently shows the feasibility of the method, and the result shows that this method is much advantageous over traditional methods in stability.Lastly, the work of the dissertation and the logical control method are briefly summarized.更多还原