肾舒Ⅱ号对阿霉素诱导的肾病综合征大鼠模型的实验研究

【作者】 毕丽娟；

【导师】 王小琴；

【作者基本信息】 湖北中医学院 ， 中医内科学， 2003， 硕士

【摘要】 目的： 探讨肾舒Ⅱ号对肾病综合征大鼠的治疗作用及对凝血机制的影响。 方法： 将Wistar雄性大鼠60只，随机分为正常组(n=12)、模型组(n=12)、肾舒Ⅱ号组(n=12)、肾舒Ⅱ号+激素组(n=12)，激素+潘生丁组(n=12)。按Bertani法制作肾病综合征模型。造模后一周开始灌胃给药，疗程为三周。采用24小时尿蛋白定量、血清白蛋白、总蛋白、甘油三酯、胆固醇、尿素氮、血肌酐作为观察指标，观察肾舒Ⅱ号对肾病综合征模型的治疗作用；采用D-二聚体及血小板α-颗粒膜蛋白为观察指标，观察肾舒Ⅱ号对凝血机制的影响；采取肾组织做光镜、电镜，观察肾脏组织结构的变化。 结果： 结果表明盐酸阿霉素尾静脉注射后7天，造模组大鼠24小时尿蛋白定量较正常组显著增加，提示肾病综合征模型制成。 给药三周后，各治疗组与模型组比较，大鼠血浆白蛋白、总蛋白含量均升高(p＜0.01)，24小时尿蛋白定量、甘油三酯，胆固醇、D-二聚体及血小板α-颗粒膜蛋白含量下降(p＜0.05，p＜0.01)。病理结果显示大鼠肾小球基底膜未见增厚，系膜无明显增生，足突仅少部分融合，与模型组比较有显著性差异。肾舒Ⅱ号+激素组与其它治疗组比较，在升高血浆总蛋白、白蛋白含量，降低24小时尿蛋白定量、甘油三脂、胆固醇含量方面有显著性差异(p＜0.05)，提示肾舒Ⅱ号+激素组的疗效优于其它治疗组。肾舒II号组在降低D-二聚体及血小板α-颗粒膜蛋白含量方面，与其它治疗组比较有显著性差异(p＜0.01)。 结论： 本研究结果表明，①用盐酸阿霉素尾静脉注射能成功诱导大鼠肾病综合征模型；②各治疗组对肾病综合征大鼠均有一定的治疗作用；③肾舒Ⅱ号+激素组治疗效果明显优于其它各组；④肾舒Ⅱ号在降低D-二聚体及血小板α-颗粒膜蛋白含量方面，明显优于其它各组。提示肾舒Ⅱ号可能通过改善凝血机制，提高对肾病综合征的疗效。更多还原

【Abstract】 Objective:To study theraputic effects and mechanism of shenshu II on Adrianmycin induced nephrotic syndrome(NS) in rats.Methods:Sixty male Wistar rats were selected and randomly divided into two groups.Twelve rats were used as normal group,and the remaining rats were given intravenouse injection of Adrianmycin (6.0mg/kg ) to establish model of NS.The model of NS rats were divided into four groups: the model group(n=12), shenshu II group (n=12) treated with shenshu II alone , shenshu II and prednisone(n=12) treated with shenshu II combined with prednisone, prednisone and persantine group(n=12)treated with prednisone combined with persantine for 21 days respectively. Normal group and the model group were given 0.9% sodium chloride. Three weeks after treatment ,the general condition of rats,24h urine protein quantity, serum albumin,totalalbumin, plasma creatinine, urea nitrogen ,cholesterol , triglyceride, D-Dimer ,GMP-140 and pathological changes of renal tissues were observed.Result:24 hour urine protein quantity increased significantly 7 days after the injection of Adriamycin .After 21 days treatment,groups treated showed significant decreasing in 24 hour urine protein quantity, cholesterol triglyceride, D-dimer, GMP-140, and increasing serum albumin ,total albumin(p<0.01,versus the model group).Shenshu II combined with prednisone got better theraputic effects(p<0.05, p<0.01,compared with the other treatments groups). D-Dimer,GMP-140 in the group treated with Shenshu II decreased significantly (p<0.01,compared with the other treatment groups). Pathological studies showed obvious fusion of footprotrusion without marked thickening of glomercular basement membrance in the model group. The fusion of partial footprotrusion was observed in the groups treated.Conclusion:The results showed:(1)the nephrotic syndrome models were induced by intravenouse injection of Adrianmycin, (2) all treated groups could get the theraputic effects,(3) the theraputic effets in the Shenshu II combined with prednisone were better than the others, (4) D-Dimer,GMP-140 in the group treated with Shenshu II decreased significantly compared with the other treated groups,which suggested the treatment with Shenshu II could get better theraputic effets on nephrotic syndrome by improving the coagulation conditions.更多还原