【作者】 向勤锃；

【导师】 刘德华；

【作者基本信息】 湖南农业大学 ， 茶学， 2003， 硕士

【摘要】 本研究对湖南农业大学实验茶场的4个茶树品种(品系)的含氟量和含铝量及蛋白质进行了研究。结果表明： 1．不同时期、不同叶位茶树鲜叶的含氟量及含铝量的差异均达极显著水平(P＜0.01)；不同品种(品系)的含氟量存在极显著的差异(P＜0.01)，但含铝量差异在所测品种(品系)间在第Ⅲ批的采摘样中达到显著水平(P＜0.05)，而在第Ⅰ、Ⅱ、Ⅳ批采摘样中未达到显著水平(P＞0.05)。不同叶位及时期采摘的鲜叶，氟、铝含量的变化规律相似：随着叶位的降低，其含量增加；均以第Ⅱ批采摘的样品含量最高，但品种间存在差异。蛋白质的SDS-PAGE分析表明，在研的四个茶树品种(品系)蛋白质谱带条数不存在差异，只存在相同Rf值的蛋白质谱带染色强度的差异；夏季的谱带染色较春季深；芽与叶的蛋白质谱带在条数及染色强度上都存在较大的差异。 2．安化圆叶组培小苗在不同浓度氟、铝元素胁迫培养下，对其是否成活或长势的综合评分的结果表明，氟、铝元素的添加，即使是微量浓度都会对小苗初代培养的生长造成明显的抑制。方差分析结果显示，在添加不同浓度氟元素的胁迫培养下，小苗褐变死亡及生长的差异达极显著水平(P＜0.01)。而不同浓度铝元素胁迫培养下，小苗生长变化的差异未达到显著水平(P＞0.05)。但茶树小苗在氟、铝胁迫培养一段时间后，能产生一定的适应性，接触培养基的未褐变死亡的芽或组织可恢复生长。氟、铝胁迫下组培苗的蛋白质分析表明，培养基只添加氟处理的各谱带染色比较深，培养至50d时，该处理与大田栽植的母株鲜叶均出现谱带40、41，而添加氟、铝胁迫培养的小苗与对照不存在谱带40、41，只添加铝胁迫培养的小苗则只有谱带41。似乎氟、铝胁迫培养，在一定的浓度范围内能使茶树体内产生免疫反应——产生小分子量的蛋白质。更多还原

【Abstract】 F content, Al content and analyses of proteins in 4 cultivars of Camellia Sinensis (L.) were conducted in the research. The results showed:1. There were very significant difference in both F content and Al content in the leaf samples picked from various tea leaf positions and various time (P<0. 01). The difference of F content from different cultivars also reached very (P<0.01). The difference of Al content from different cultivars reached significant only in the III samples (P<0.05). The change of Al content in the tea leaf samples from various leaf positions and various times was similar with that of F content. The content of both F and Al increased as the leaf position lowering. F content and Al content reached a peak in the IIsamples with difference from various cultivars. SDS-PAGE analyses of proteins were also conducted and the results indicated that there was difference only in dyeing intensity of proteins from various cultivars and different time. But there were difference both in dyeing intensity and number of proteins from buds and leaves.2. Young tea plants were cultured on press of various concentrations of F&A1. The results showed growths of young plants were seriously affected in first culture. Variance analysis of growth evaluation of young tea plants indicated the difference of growth and changing of young plants under various F concentration reached very significant (P<0. 01) while that of young plants under various Al concentration didn’ t reach significant (P>0. 05). These young plants produced tolerance during further culture with press of F & Al and some young plants recovered from the buds and tissues that haven’ t being brown totally or died. SDS-PAGE analyses of proteins to those young plants cultured on press of F&A1 were conducted in the experiment. The results showed that the dyeing intensity of proteins in the plants cultured in the medium added only with F is stronger than other 3 treatments when 30d past. After 50 days, two new proteins were found in the young plant from the Immol/L F treatment compared with those from basic medium and the medium added with Immol/L F and 3mmol/L Al. And one of the two proteins was found in the young tea plant from the treatment with 3 mmol/L Al in the medium. Both of the two proteins were found in leaves of culturing tea plant-Anhua Yuanye. It seemed that tea plants could become immune to the press of F&A1 in a certain range of F&A1 concentration just because they had produced new proteins with small molecular weight.更多还原